北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 儿科  > 期刊论文
学科主题: 儿科学
题名:
561例新生儿胰岛素样生长因子-1启动子区域基因多态性观察
其他题名: Characteristics of polymorphism in promoter region of insulin-like growth factor-1 gene in 561 neonates
作者: 王晴晴; 朴梅花; 王新利; 韩彤妍; 张娟; 关育红; 齐婧
关键词: 胰岛素样生长因子Ⅰ ; 启动区(遗传学) ; 多态现象,遗传 ; 婴儿,新生 ; Insulin-like growth factor Ⅰ ; Promoter regions (genetics) ; Polymorphism,genetic ; Infant,newborn
刊名: 中华围产医学杂志
发表日期: 2013
DOI: 10.3760/cma.j.issn.1007-9408.2013.05.004
卷: 16, 期:5, 页:274-277
收录类别: 中国科技核心期刊 ; 中文核心期刊
文章类型: Journal Article
摘要: 目的 检测新生儿胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)启动子区域737/738位点胞嘧啶腺苷(cytosine-adenosine,CA)重复序列的基因多态性. 方法 选择2010年6月1日至2012年6月30日于北京大学第三医院出生的健康新生儿561例.记录新生儿的性别、出生胎龄、出生体重、身长,并进行胎龄评估.出生后3~5 d清晨空腹采集足跟血,提取DNA,分析IGF-1启动子区域基因多态性.采用x2检验、独立样本t检验、方差分析和Hardy-Weinberg平衡检验进行统计学分析. 结果 561例新生儿中,足月儿413例,早产儿148例;大于胎龄儿92例,适于胎龄儿433例,小于胎龄儿36例.561例共发现7种不同的等位基因及23种不同的基因型.7种等位基因分别为188、190、192、194、196、198和200 bp.最常见的3种基因型为190~192 bp、192~196 bp及192~192 bp,其频率分别为23.2%(130/561)、15.0%(84/561)和12.8%(72/561).CA19纯合基因型(CA19/CA19)、CA19杂合基因型(CA19/CAno19)及CA19突变基因型(CAno19/CAno19)的频率在足月儿和早产儿之间差异均无统计学意义[分别为11.4%(47/413)与16.9%(25/148)、55.9%(231/413)与50.7%(75/148)、32.7%(135/413)与32.4%(48/148),x2=2.96、1.21和0.00,P均>0.05].CA19/CA19、CA19/CAno19、CAno19/CAno19这3种基因型新生儿的出生胎龄分别为(37.1±2.9)、(37.6±3.1)和(37.4±3.1)周,差异无统计学意义(F=0.54,P=0.58).CA19/CA19在小于胎龄儿中的频率明显高于大于胎龄儿及适于胎龄儿[分别为25.0%(9/36)、7.6%(7/92)及12.9%(56/433),x2=7.01,P=0.03],但CA19/CAno19及CAno19/CAno19基因型频率在大于胎龄儿、适于胎龄儿及小于胎龄儿3组之间差异无统计学意义(CA19/CAno19:x2=1.13,P=0.57;CAno19/CAno19:x2=0.58,P=0.75). 结论 IGF-1启动子区存在基因多态性,CA19等位基因频率与出生胎龄无关. Objective To investigate polymorphism in the promoter region of insulin-like growth factor-1(IGF-1) gene.Methods Five hundred and sixty-one neonates admitted to Peking University Third Hospital from June 1st,2010 to June 30th,2012 were recruited into the study.Gender,gestational age,birth weight and birth length were collected.Heel blood samples were collected on 3-5 days after birth.DNA was extracted to analyze the polymorphism in the promoter region of IGF-1 gene.Chi-square test,independent-sample t-test,analysis of variance and HardyWeinberg equilibrium were performed.Results Among the 561 neonates,413 were full term,and 148 were preterm; 92 were large for gestational age (LGA),433 were appropriate for gestional age (AGA),and 36 were small for gestional age (SGA).Seven different alleles and 23 genotypes in the promoter region of IGF-1 gene were identified in the population.The seven alleles were 188,190,192,194,196,198 and 200 bp respectively.The three most common genotypes were 190-192 bp,192-196 bp and 192-192 bp,whose frequencies were 23.2% (130/561),15.0% (84/561) and 12.8%(72/561).There were no significant differences of cytosine-adenosine (CA)19/CA19,CA19/CAno19and CAno19/CAno19 genotypes between full term and preterm infants [11.4% (47/413) vs 16.9%(25/148),55.9% (231/413) vs 50.7% (75/148) and 32.7% (135/413) vs 32.4% (48/148)respectively,x2=2.96,1.21 and 0.00,all P>0.05].There was no difference in the gestional age among infants with CA19/CA19,CA19/CAno19 and CAno19/CAno19 genotypes [(37.1±2.9),(37.6±3.1) and (37.4±3.1) weeks respectively,F=0.54,P=0.58].The frequency of CA19/CA19 in SGA neonates was higher than that in LGA and AGA neonates [25.0% (9/36) vs 7.6%(7/92) and 12.9% (56/433),x2 =7.01,P=0.03],but there were no differences in the frequency of CA19/CAno19 and CAno19/CAno19 among LGA,AGA and SGA neonates (CA19/CAno19:x2 =1.13,P=0.57; CAno19/CAno19:x2 =0.58,P=0.75).Conclusions Polymorphism exists in the promoter region of IGF-1 gene.The gestational age is not associated with the frequency of CA19 allele.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68864
Appears in Collections:北京大学第三临床医学院_儿科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 100191,北京大学第三医院儿科

Recommended Citation:
王晴晴,朴梅花,王新利,等. 561例新生儿胰岛素样生长因子-1启动子区域基因多态性观察[J]. 中华围产医学杂志,2013,16(5):274-277.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[王晴晴]'s Articles
[朴梅花]'s Articles
[王新利]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[王晴晴]‘s Articles
[朴梅花]‘s Articles
[王新利]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace