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叶酸和维生素B12对胎儿生长受限大鼠胰岛素抵抗的影响
其他题名Effects of folic acid and vitamin B12 on insulin resistance of fetal growth restriction rats
张慧; 邢燕; 张金; 王新利; 朴梅花; 童笑梅; 韩彤妍
关键词胎儿生长迟缓 胰岛素抗药性 叶酸 维生素B12 大鼠,Sprague-Dawlev Fetal growth retardation Insulin resistance Folic acid Vitamin B12 Rats,Sprague-Dawley
刊名中华围产医学杂志
2015
DOI10.3760/cma.j.issn.1007-9408.2015.08.008
18期:8页:589-593
收录类别中国科技核心期刊 ; 中文核心期刊
文章类型Journal Article
摘要目的 探讨在哺乳期母鼠的食物中添加叶酸和维生素B12对胎儿生长受限(fetal growth restriction,FGR)仔鼠胰岛素抵抗的影响. 方法 18只Sprague-Dawley雌鼠与雄鼠交配,受孕第12天随机分为低蛋白组(12只)和对照组(6只).孕鼠自然分娩,低蛋白组中FGR仔鼠共84只(93.3%,84/90),随机抽取48只分为FGR干预组和FGR未干预组(各24只).干预组的哺乳期母鼠饲以高叶酸和维生素B12的饲料,未干预组和对照组的哺乳期母鼠饲以普通饲料.3组仔鼠均于生后21d断奶,饲以普通饲料.于仔鼠生后21、60和120 d,每组随机选择8只仔鼠,测定血浆葡萄糖(简称血糖)、血清胰岛素、甘油三酯和胆固醇水平,并计算胰岛素敏感指数(insulin sensitivity index,ISI)和胰岛素抵抗指数(insulin resistance index,IRI).采用方差分析、Student-Newman-Keuls法进行统计分析. 结果 (1)FGR发生率:低蛋白组仔鼠平均出生体重[(4.44±0.58)g]低于对照组[(7.03±0.56)g],差异有统计学意义(t=15.75,P<0.05).(2)血糖和胰岛素水平:生后21d,FGR未干预组、干预组和对照组的空腹血糖(fasting plasmaglucose,FPG)分别为(4.8±0.3)、(4.8±0.4)与(4.6±0.3)mmol/L (F=0.57),空腹胰岛素(fasting insulin,FINS)分别为(4.2±0.2)、(4.5±0.4)与(4.3±0.1)mU/L(F=0.31),差异均无统计学意义(P值均> 0.05).生后60 d,3组FPG分别为(7.5±0.4)、(6.9±1.0)与(5.5±0.6) mmol/L(F=17.14),FINS分别为(14.7±1.9)、(10.7±1.0)与(9.2±0.7) mU/L(F=38.34);生后120 d,3组FPG分别为(8.9±0.9)、(8.0±0.8)与(6.4±0.7)mmol/L(F=21.60),FINS分别为(56.3±2.8)、(38.2±2.5)与(33.1±2.8)mU/L(F=164.46);FGR未干预组最高,对照组最低,差异均有统计学意义(P值均< 0.05).(3)IRI和ISI:生后21d,FGR未干预组、干预组和对照组的IRI分别为0.9±0.1、0.9±0.1与0.9±0.2(F=0.49),ISI分别为-(3.0±0.7)、-(3.0±0.1)与-(3.0±0.3)(F=0.69),差异均无统计学意义(P值均>0.05).生后60d,3组IRI分别为4.9±0.8、3.3±0.3与2.2±0.3(F=49.48),ISI分别为-(4.7±0.2)、-(4.3±0.1)与-(3.9±0.1) (F=63.47);生后120 d,3组IRI分别为22.4±3.1、13.6±2.0与9.3±0.9(F=75.15),ISI分别为-(6.2±0.1)、-(5.7±0.1)与-(5.3±0.1)(F=104.42),差异均有统计学意义(P值均< 0.05).(4)胆固醇和甘油三酯:生后21 d,FGR未干预组、干预组和对照组的胆固醇分别为(2.0±0.1)、(2.0±0.1)与(2.0±0.1)mmol/L (F=0.10),甘油三酯分别为(0.8±0.1)、(0.8±0.1)与(0.7±0.1)mmol/L(F=0.33),差异均无统计学意义(P值均>0.05).生后60 d,3组胆固醇分别为(2.3±0.1)、(2.2±0.1)与(2.0±0.2) mmol/L(F=8.34),甘油三酯分别为(1.5±0.2)、(1.2±0.1)与(1.0±0.2) mmol/L (F=17.93);生后120 d,3组胆固醇分别为(2.4±0.2)、(2.2±0.1)与(2.1±0.1) mmol/L (F=6.12),甘油三酯分别为(1.7±0.5)、(1.2±0.3)与(1.0±0.1)mmol/L(F=9.80);FGR未干预组最高,对照组最低,差异均有统计学意义(P值均< 0.05). 结论 母鼠哺乳期补充叶酸和维生素B12,能一定程度上改善FGR仔鼠的胰岛素抵抗,但并不足以完全修复糖脂代谢. Objective To explore the effects of folic acid and vitamin B12 supplement in maternal lactation on insulin resistance in fetal growth restriction (FGR) in rat offspring.Methods Eighteen Sprague-Dawley female rats and male rats were used.Pregnant rats were randomly divided into two groups at 12 days:normal-protein group (NP,n=6) and low-protein group (LP,n=12).The were 84 FGR newborn pups in LP group (93.3%,84/90).Forty-eight FGR newborn pups were randomly selected and divided into two groups (24 in each group):intervention group and non-intervention group.The intervention group was fed with high folate and vitamin B12 in the diet;and non-intervention group and NP group were fed normal diet.All of the newborn pups were weaned at 21 days after birth and then fed with normal diet.At days 21,60 and 120 d after birth,eight pups were randomly selected from each group and fasting plasma glucose (FPG),fasting insulin (FINS),blood diglyceride (TG) and cholesterol (TC) were measured.The insulin resistance index (IRI) and insulin sensitivity index (ISI) were calculated to evaluate insulin sensitivity.Variance and Student-Newman-Keuls tests were used for statistical analysis.Results (1) The incidence of FGR:Birth weight of LP offspring [(4.44±0.58) g] was significantly lower than that of NP ones [(7.03±0.56) g] (t=15.75,P < 0.05).(2) FPG and FINS:at day 21 after birth,FPG of the non-intervention group,intervention group and NP group was (4.8±0.3),(4.8±0.4) and (4.6±0.3) mmol/L (F=0.57),respectively;FINS was (4.2± 0.2),(4.5 ±0.4) and (4.3 ±0.1) mU/L (F=0.31),respectively;and there was no significant difference among the three groups (both P > 0.05).At day 60,FPG of the three groups was (7.5±0.4),(6.9± 1.0) and (5.5±0.6) mmol/L (F=17.14);FINS was (14.7± 1.9),(10.7± 1.0) and (9.2± 0.7) mU/L (F=38.34),respectively.At day 120,FPG was (8.9±0.9),(8.0±0.8) and (6.4±0.7) mmol/L (F=21.60);FINS was (56.3±2.8),(38.2±2.5) and (33.1 ±2.8) mU/L (F=164.46).FPG and FINS were highest in the non-intervention group,and lowest in NP group,with significant differences among the three groups of pups (all P < 0.05).(3) IRI and ISI:at day 21,IRI of the non-intervention group,intervention group and the control group was 0.9±0.1,0.9±0.1 and 0.9±0.2 (F=0.49);ISI was-(3.0±0.7),-(3.0±0.1) and-(3.0±0.3) (F=0.69);and there was no significant difference among the three groups (both P > 0.05).At day 60,IRI of the three groups was 4.9±0.8,3.3±0.3 and 2.2±0.3 (F=49.48);ISI was-(4.7±0.2),-(4.3±0.1) and-(3.9±0.1) (F=63.47).At day 120,IRI of the three groups was 22.4±3.1,13.6±2.0 and 9.3±0.9 (F=75.15);ISI was -(6.2 ± 0.1),-(5.7 ± 0.1) and-(5.3 ± 0.1) (F=104.42);and there were significant differences among the three groups (all P < 0.05).(4) TC and TG:at day 21,TC of the non-intervention group,intervention group and the control group was (2.0±0.1),(2.0±0.1) and (2.0±0.1) mmol/L (F=0.10);TG was (0.75±0.1),(0.77±0.1) and (0.74±0.1) mmol/L (F=0.33);and there was no significant difference among the three groups (both P > 0.05).At day 60,TC of the three groups was (2.3 ± 0.1),(2.2 ± 0.1) and (2.0± 0.2) mmol/L (F=8.34);TG was (1.5 ± 0.2),(1.2±0.1) and (1.0±0.2) mmol/L (F=17.93).At day 120,TC was (2.4±0.2),(2.2±0.1) and (2.1 ±0.1) mmol/L (F=6.12);TG was (1.7±0.5),(1.2±0.3) and (l.0±0.1) mmol/L (F=9.80).The TC and TG were highest in the non-intervention group and the lowest in the control group;and there were significant differences among the three groups (all P < 0.05).Conclusion Supplementing folic acid and vitamin B12 in maternal lactation can improve in some extent insulin resistance in FGR rats,but not sufficient enough to completely repair glucose and lipid metabolism.
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68889
Collection北京大学第三临床医学院_儿科
作者单位100191,北京大学第三医院儿科
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张慧,邢燕,张金,等. 叶酸和维生素B12对胎儿生长受限大鼠胰岛素抵抗的影响[J]. 中华围产医学杂志,2015,18(8):589-593.
APA 张慧.,邢燕.,张金.,王新利.,朴梅花.,...&韩彤妍.(2015).叶酸和维生素B12对胎儿生长受限大鼠胰岛素抵抗的影响.中华围产医学杂志,18(8),589-593.
MLA 张慧,et al."叶酸和维生素B12对胎儿生长受限大鼠胰岛素抵抗的影响".中华围产医学杂志 18.8(2015):589-593.
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