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学科主题: 放射科学
题名:
Gd-DTPA磁共振成像示踪法对脑细胞外间隙成像与定量分析的初步研究
其他题名: Imaging and quantitative measurement of brain extracellular space using MRI Gd-DTPA tracer method
作者: 和清源; 韩鸿宾; 许方婧伟; 闫军浩; 曾津津; 李小刚; 傅瑜; 彭芸; 陈翯; 侯超; 徐晓娟
关键词: 磁共振成像 ; 细胞外隙 ; 钆喷酸葡胺 ; Magnetic resonance imaging ; Extracellular space ; Gadolinium DTPA
刊名: 北京大学学报(医学版)
发表日期: 2010
DOI: 10.3969/j.issn.1671-167X.2010.02.016
卷: 42, 期:2, 页:188-191
文章类型: Journal Article
摘要: 目的:观察钆喷酸葡胺(Gd-DTPA)在脑细胞外间隙(brain extracellular space,ECS)中扩散的磁共振成像(magnetic resonance imaging,MRI)表现与规律,探讨应用MRI示踪法对ECS进行定量分析的可行性.方法:将8只雄性SD大鼠(280 g~320 g)麻醉后,参考脑立体定位图谱,选取冠状位苍白球为中心层面,以尾状核为靶点进行穿刺定位,导入Gd-DTPA 2 μL.于导入后1、3、6、9、12 h分别进行MRI扫描,观察MRI表现,测量示踪剂导入侧所在脑区与对侧相应脑区MR信号强度随时间的变化规律.于12 h MRI扫描结束后,对大鼠进行行为学评分.结果:MRI图像证实6只大鼠Gd-DTPA导入点位于尾状核区,2只位于外囊区.Gd-DTPA在尾状核区导入后呈均匀扩散,3 h扩散到达同侧大脑皮层,扩散后主要分布在大脑中动脉供血区的脑组织.随着扩散范围的增大,尾状核区的MRI信号强度逐渐下降,3 h后各个时间点相对MRI信号强度△s与1 h时△s间差异存在统计学意义(t=95.63,P<0.01),3 h后的各组△S间差异无统计学意义(P>0.05).△s的衰减规律呈指数形式[Y=1.7886x~(-0.1776)(R~2=0.94)].导入外囊区的Gd-DTPA在1 h的MRI上呈弧形沿自质纤维扩散,表现为各向异性.在3 h后呈扇形向相邻脑区扩散,6 h时扩散到达同侧皮层区.结论:应用MRI示踪法可动态观察Gd-DTPA在细胞外间隙的扩散与清除衰减规律,是在活体对ECS解剖与生理学参数进行定量研究的可行方法. Objective:To observe the diffusion of Gd-DTPA in brain extracellular space (ECS) by magnetic resonance imaging (MRI) and investigate the feasibility of ECS measurement by using MRI tra cer method in vivo.Methods:2 μL Gd-DTPA was introduced into ECS by caudate nucleus according to stereotaxic atlas in 8 Sprague Dawley (SD) rats (male,280~320 g).The MRI scans were performed at 1 h,3 h,6 h,9 h and 12 h respectively after administration.MRI appearances of Gd-DTPA diffusion and distribution was observed and compared.The MRI signal enhancement was measured at each time point.The neuroethology assessment was performed after MRI scanning at 12 h.Results:The injection was accurate at the center of the caudate nucleus in 6 rats,while,at the capsula externa in other 2 rats.Gd-DTPA diffused isotropically after it was introduced into caudate nucleus,which spread into lateral cor-tex at 3 h.The MRI signal enhancement distributed mainly in the middle cerebral artery territory.A sig-nificant difference was found between the signal enhancement ratio at 1 h and that at 3 h in the original point of caudate nucleus(t=95.63,P<0.01),and the signal enhancement attenuated following the ex-ponential power function y=1.7886x~(-0.1776) (R~2=0.94).In 2 rats with the injection point at capsula ex-terna,Gd-DTPA diffused anisotropically along the fiber track of white matter during 1 h to 3 h,and spread into the lateral cortex at 6 h.Conclusion:The diffusion and clearance of Gd-DTPA in brain ECS could be monitored and measured quantitatively in vivo by MRI tracer method.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/69546
Appears in Collections:北京大学第三临床医学院_放射科_期刊论文

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作者单位: 1.北京大学第三医院放射科,北京,100191
2.北京大学基础医学院解剖学教研室
3.北京儿童医院放射科
4.北京大学第三医院神经科

Recommended Citation:
和清源,韩鸿宾,许方婧伟,等. Gd-DTPA磁共振成像示踪法对脑细胞外间隙成像与定量分析的初步研究[J]. 北京大学学报(医学版),2010,42(2):188-191.
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