北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 风湿免疫科  > 期刊论文
学科主题: 免疫学
题名:
鼠科肉瘤病毒癌基因同源物B1蛋白在类风湿关节炎滑液中的表达及其临床意义
其他题名: Detection of BRAF in the synovial fluid and its clinical significance in rheumatoid arthritis
作者: 赵金霞; 孙琳; 张颖健; 张霞; 李茹; 刘湘源
关键词: 关节炎,类风湿 ; 滑液 ; 鼠科肉瘤病毒癌基因同源物B1 ; Arthritis,rheumatoid ; Synovial fluid ; v raf murine sarcoma viral oncogene homologue B1
刊名: 中华风湿病学杂志
发表日期: 2013
DOI: 10.3760/cma.j.issn.1007-7480.2013.07.005
卷: 17, 期:7, 页:446-449
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 检测类风湿关节炎(RA)患者滑液中鼠科肉瘤病毒癌基因同源物B1(BRAF)蛋白水平,探讨其在RA中的临床意义.方法 应用酶联免疫吸附法(ELISA)检测44例RA患者及22例骨关节炎患者滑液中BRAF蛋白水平,同时检测25例RA、12例骨关节炎以及21名健康对照血清中BRAF蛋白水平.应用蛋白印迹法检测RA患者和骨关节炎患者滑膜组织中BRAF蛋白表达水平.收集RA患者的临床和实验室资料,评价RA患者滑液中BRAF蛋白与临床和实验室指标的关系.采用t检验和Spearman相关性分析进行统计学分析.结果 ①RA患者滑液中BRAF蛋白浓度为[(84±59) ng/ml],明显高于骨关节炎组[(38±41) ng/ml,t=3.290,P=0.002].②RA患者血清中BRAF蛋白水平[(22.0±12.5)ng/ml]亦明显高于骨关节炎组[(6.8±7.5) ng/ml,t=3.882,P<0.01]和健康对照组[(4.8±2.2) ng/ml,t=6.766,P<0.01].③8例RA患者配对的滑液和血清中BRAF蛋白检测结果显示,BRAF蛋白在RA患者滑液中的表达水平[(102±52) ng/ml]明显高于血清[(21±12) ng/ml,t=-4.316,P=0.003].④RA患者滑膜组织中BRAF蛋白表达水平(0.284±0.045)高于骨关节炎患者(0.191±0.013,t=3.169,P=0.034).⑤RA患者滑液中BRAF蛋白与病程呈负相关(r=-0.40,P=0.019),与类风湿因子呈正相关(r=0.37,P=0.03),与其他临床和实验室指标之间无相关性.结论 RA患者滑液和滑膜组织中有BRAF蛋白的表达,且滑液中的表达水平较血清中明显升高,提示BRAF蛋白可能在RA发病的早期阶段发挥作用. Objective To detect v raf murine sarcoma viral oncogene homologue B1 (BRAF) in the synovial fluid of rheumatoid arthritis (RA) and to investigate its clinical significance in RA.Methods Synovial fluid samples were obtained from patients with RA and osteoarthritis (OA).Serum samples were obtained from patients with RA,OA and heathy controls.The presence of BRAF in the synovial fluid and sera were examined by enzyme-linked immunosorbent assay (ELISA).Western blotting was used to detect the expression of BRAF protein in the synovial tissue of RA and OA.The associations between the BRAF and the clinical features and laboratory parameters of RA were evaluated.Data analysis were performed using t test and Spearman's association analysis.Results ① The level of BRAF in the synovial fluid of RA [(84±59) ng/ml] was significantly higher than OA [(38±41) ng/ml] (t=3.290,P=0.002).② The level of BRAF in the sera of RA patients [(22.0±12.5) ng/ml] was also higher than OA [(6.8±7.5) ng/ml,t=3.882,P<0.01] and healthy controls [(4.8±2.2) ng/ml,t=6.766,P<0.01].③ In RA patients,the BRAF protein level in the synovial fluid [(102±52) ng/ml] was significantly higher than that in the serum [(21±12) ng/ml] (t=-4.316,P=0.003).④The expression level of BRAF in the synovial tissue of RA (0.284±0.045) was higher than that in OA patients (0.191±0.013,t=3.169,P=0.034).⑤ The level of BRAF in the synovial fluid had a negative correlation with disease duration (r=-0.40,P=0.019) and a positive correlation with rheumatoid factor (RF) levels (r=0.37,P=0.03).Conclusion The presence of BRAF in the synovial fluid and synovium of RA indicates that BRAF may play a role in the pathogenesis of RA,especially in the early stage.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/69650
Appears in Collections:北京大学第三临床医学院_风湿免疫科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.100191,北京大学第三医院风湿免疫科
2.北京大学人民医院风湿免疫科

Recommended Citation:
赵金霞,孙琳,张颖健,等. 鼠科肉瘤病毒癌基因同源物B1蛋白在类风湿关节炎滑液中的表达及其临床意义[J]. 中华风湿病学杂志,2013,17(7):446-449.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[赵金霞]'s Articles
[孙琳]'s Articles
[张颖健]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[赵金霞]‘s Articles
[孙琳]‘s Articles
[张颖健]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace