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IR@PKUHSC  > 北京大学第三临床医学院  > 风湿免疫科  > 期刊论文
学科主题: 免疫学
题名:
CXCL16在胶原诱导性关节炎小鼠中的表达及对淋巴细胞增殖活化的影响
其他题名: Expression of chemokine CXCL16 in murine collagen-induced arthritis and the effects on the proliferation of lymphocytes
作者: 孙琳; 刘湘源; 赵金霞; 满斯亮; 张霞
关键词: 趋化因子 ; 关节炎,实验性 ; 淋巴细胞 ; Chemokine ; Arthritis,experimental ; Lymphocytes
刊名: 中华风湿病学杂志
发表日期: 2011
DOI: 10.3760/cma.j.issn.1007-7480.2011.03.003
卷: 15, 期:3, 页:151-154
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 了解CXCL16在胶原诱导性关节炎(CIA)小鼠发病中的作用.方法 以8只正常小鼠为对照,采用酶联免疫吸附试验(ELISA)和反转录-聚合酶链反应(RT-PCR)法,分别检测CXCL16在12只CIA小鼠外周血中的蛋白水平及病变滑膜组织中的mRNA水平,再采用CCK-8法、RT-PCR和ELISA,分别检测不同浓度的CXCL16重组蛋白(0,100,200,400,800 ng/ml)刺激鼠脾淋巴细胞的增殖情况、上清中白细胞介素(IL)-2和干扰素-γ水平及RANKL mRNA表达水平.采用t检验和单因素方差分析进行统计分析.结果 ①与对照组相比,CIA小鼠外周血CXCL16[分别为(72±8)和(127±10)pg/ml,P<0.05]和滑膜CXCL16 mRNA水平(分别为0.214±0.007和0.375±0.009,P<0.01)均显著增高.②CXCL16(200、400、800 ng/ml)作用于CIA小鼠淋巴细胞,其增殖能力明显高于CXCL16(0 ng/ml),吸光度(A)值分别为0.51±0.06、0.56±0.05、0.55±0.04、0.41±0.04(P<0.05).并且CXCL16对CIA组小鼠淋巴细胞的增殖刺激能力明显高于其对健康对照组小鼠淋巴细胞(P均<0.05).③核因子κB受体活化因子配体(RANKL)mRNA、IL-2和干扰素-γ水平在CIA CXCL16(400、800 ng/ml)组均明显高于CXCL16(0 ng/ml)组(P<0.01).结论 CXCL16可能通过活化淋巴细胞在CIA发病中发挥重要作用. Objective To investigate the effect of CXCL16 on the development of murine collageninduced arthritis (CIA). Methods CXCL16 mRNA of the involved synovium and serum CXCL16 protein were determined respectively by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA) in murine collagen-induced arthritis. The proliferation of lymphocytes from murine spleen and the level of RANKL mRNA, stimulated by CXCL16 at different concentrations (0,100, 200, 400, 800 ng/ml), was detected respectively by CCK-8 and RT-PCR, then the level of IL-2 and IFN-γ in culture supernatant was detected by ELISA. Comparisons between groups were tested by t test and one-way ANOVA analysis. Results The serum CXCL16 [(127± 10) vs (72±8) pg/ml, P<0.05] and synovial CXCL16 mRNA (0.214±0.007 vs 0.375±0.009, P<0.01) in CIA were all significantly higher than those in normal controls. The proliferation of CXCL16 (200, 400, 800 ng/ml) in CIA mouse lymphocytes, was significantly higher than that of CXCL16 (0 ng/ml) (0.51±0.06, 0.56±0.05, 0.55±0.04, (0.41±0.04, P<0.05). And CXCL16 on the CIA stimulated lymphocyte proliferation was significantly higher than controls on normal lymphocytes (P<0.05). Compared with blank control group, the expression of IL-2, IFN-γ and RANKL mRNA of CIA CXCL16 (400, 800 ng/ml) groups was higher significantly (P<0.01). Conclusion CXCL16 plays an important role in the development of murine CIA by activating lymphocytes.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/69679
Appears in Collections:北京大学第三临床医学院_风湿免疫科_期刊论文

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作者单位: 1.北京大学第三医院风湿免疫科,100191
2.北京市积水潭医院风湿免疫科

Recommended Citation:
孙琳,刘湘源,赵金霞,等. CXCL16在胶原诱导性关节炎小鼠中的表达及对淋巴细胞增殖活化的影响[J]. 中华风湿病学杂志,2011,15(3):151-154.
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