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学科主题: 传染病学
题名:
小鼠肺泡巨噬细胞中腺苷酸活化蛋白激酶参与内毒素诱导炎症因子分泌的调控
其他题名: Role of AMP-activated protein kinase in regulating LPS-induced secretion of inflammatory cytokines in mouse alveolar macrophages
作者: 王伟; 李晓光; 胥婕
关键词: 小鼠肺泡巨噬细胞 ; 腺苷酸活化蛋白激酶 ; 脂多糖 ; 炎症因子 ; Mice alveolar macrophage ; Adenosine monophosphate-activated protein kinase ; LPS ; Inflammatory cytokine
刊名: 中华微生物学和免疫学杂志
发表日期: 2015
DOI: 10.3760/cma.j.issn.0254-5101.2015.07.005
卷: 35, 期:7, 页:502-505
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨腺苷酸活化蛋白激酶( AMP-activated protein kinase, AMPK)在小鼠肺泡巨噬细胞由脂多糖( lipopolysaccharide,LPS)诱导炎症因子分泌中的作用机制. 方法 选择野生型和AMPKα1-/-小鼠原代肺泡巨噬细胞,ELISA(酶联免疫吸附剂测定)方法检测在有无AMPK的激动剂[5-氨基-4-甲酰胺咪唑核糖核苷酸(5-amino-1-β-D-ribofuranosyl-imidazole-4-carboxamide,AICAR)]作用下LPS刺激的细胞上清中肿瘤坏死因子-α( TNF-α)、白细胞介素-1β( IL-1β)分泌;Western blot方法检测AMPKα1、α2在野生型和AMPKα1-/-小鼠肺泡巨噬细胞中的表达, LPS作用下p-AMPKα活性变化. 结果 AMPKα1-/-小鼠肺泡巨噬细胞中LPS刺激的炎症因子分泌显著高于野生型;野生型小鼠肺泡巨噬细胞LPS下调p-AMPKα活性, AICAR显著抑制LPS诱导的TNF-α、IL-1β的分泌. 结论AMPKα1缺失促进了LPS诱导的炎症因子分泌,AMPK激动剂AICAR显著抑制LPS诱导的炎症因子分泌,AMPK参与了小鼠肺泡巨噬细胞中内毒素诱导的炎症因子分泌调控. Objective To investigate the mechanism of 5′adenosine monophosphate-activated pro-tein kinase ( AMPK) in the regulation of LPS-induced secretion of inflammatory cytokines in mouse alveolar macrophages.Methods Alveolar macrophages were isolated from wild type and AMPKα1-/-C57BL/6J mice.ELISA was used to measure the concentrations of IL-1βand TNF-αin the culture supernatants of LPS treated alveolar macrophages with or without 5-amino-1-β-D-ribofuranosyl-imidazole-4-carboxamide ( AICAR ) stimulation.Western blot assay was performed to analyze the expression of AMPKα1 and AMPKα2 in alveolar macrophages isolated from wild type and AMPKα1-/-mice as well as the LPS-induced changes of p-AMPKαactivity in wild type mice.Results The LPS-induced secretion of inflammatory cyto-kines in alveolar macrophages isolated from AMPKα1-/-mice were significantly higher than that of wild type mice.The activities of p-AMPKαin wild type mice were suppressed by LPS treatment.Treatment of AICAR inhibited the LPS-induced secretion of TNF-αand IL-1β.Conclusion The LPS-induced secretion of in-flammatory cytokines was enhanced in AMPKα1-deficient mice, but could be suppressed upon the treatment of AICAR, an agonist of AMPK.AMPK was involved in the regulation of LPS-induced secretion of inflamma-tory cytokines by alveolar macrophages in mice.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/70306
Appears in Collections:北京大学第三临床医学院_感染疾病科_期刊论文

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作者单位: 100191,北京大学第三医院感染疾病科

Recommended Citation:
王伟,李晓光,胥婕. 小鼠肺泡巨噬细胞中腺苷酸活化蛋白激酶参与内毒素诱导炎症因子分泌的调控[J]. 中华微生物学和免疫学杂志,2015,35(7):502-505.
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