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学科主题: 传染病学
题名:
未经治疗的慢性乙型肝炎患者乙型肝炎病毒耐药变异、基因型和血清型研究
其他题名: Drug resistance, genotype, and serotype of hepatitis B virus in nucleos(t)ide analogue naive patients with chronic hepatitis B
作者: 李晓光; 胥婕; 刘宝明; 杨靖娴; 闫玲; 李彤; 庄辉
关键词: 肝炎,乙型,慢性 ; 肝炎病毒,乙型 ; 变异(遗传学) ; 抗药性 ; 基因型 ; 血清分型 ; Hepatitis B,chronic ; Hepatitis B virus ; Variation (genetics) ; Drug resistance ; Genotype ; Serotyping
刊名: 中华肝脏病杂志
发表日期: 2010
DOI: 10.3760/cma.j.issn.1007-3418.2010.02.004
卷: 18, 期:2, 页:92-95
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 研究未经核苷(酸)类似物(NA)治疗的慢性乙型肝炎患者HBV耐药变异、基因型、基因亚型和血清型特点.方法 从北京大学附属医院收集97例未经NA治疗的慢性乙型肝炎患者血清,用半巢式聚合酶链反应-直接测序法获得HBV全长逆转录酶区序列,用生物信息学技术筛查该区内11个经典耐药变异位点并鉴定基因型、基因亚型和血清型.用统计分析软件SPSS11.0进行t检验和χ~2检验. 结果 HBV在11个经典耐药变异位点上均为野生型氨基酸;B基因型和C基因型分别占36.1%(35/97)和63.9%(62/97),前者均属B2亚型,后者C2亚型占91.9%(57/62),C1亚型占6.5%(4/62),1例未能分出亚型.已知出生地的患者中,71.9%(23/32) B基因型感染者出生于我国南方地区,81.6%(40/49) C基因型感染者出生于北方地区,基因型地域分布特点明显,χ~2=23.19,P<0.01.血清型为adr者占60.8%(59/97),与C基因型相关;为adw者占38.1%(37/97),与B基因型相关,χ~2=87.83,P<0.01.结论 未经NA治疗的慢性乙型肝炎患者体内野毒株为优势株,其基因型、基因亚型和血清型与患者出生地有关. Objective To investigate drug resistance, ganotype and serotype of hepatitis B virus (HBV) in nucleos(t)ide analogue (NA) naive patients with chronic hepatitis B (CHB). Methods Full-length reverse transcriptase region of HBV DNA was amplified by semi-nested polymerase chain reaction from 97 NA-naive CHB patients, and the PCR product was sequenced, and analyzed to screen 11 classical antiviral drug resistance mutation sites and to identify HBV genotypes, subgenotypes and serotypes. Results Wild-type sequences were found at all of the 11 classical antiviral drug resistance mutation sites from all samples. The patients were infected with either genotype B (36.1%, 35/97) or C (63.9%, 62/97) HBV. The former were all belonged to subgenotype B2 strain;while the latter were divided further into subgenotype C2 (91.9%, 57/62), subgenotype C1 (6.5%, 4/62) and unknown subgenotype (1.6%, 1/62). The 71.9% (23/32) of HBV genotype B patients were born in southern China, while 81.6% (40/49) of HBV genotype C patients were from northem China, showing a clear geographic distribution ( χ~2 = 23.19, P < 0.01). Of 97 CHB patients, 59 (60.8%) were serotype adr associated with genotype C, while 37 (38.1%) were adw related to genotype B (subgenotype B2) (χ~2= 87.83, P < 0.01). Conclusion The wild-type HBV strains prevail in NA-naive CHB patients, whose HBV genotypes, subgenotypes and serotypes are associated with their places of birth.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/70317
Appears in Collections:北京大学第三临床医学院_感染疾病科_期刊论文

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作者单位: 1.北京大学第三医院感染疾病科,100191
2.北京大学医学部病原生物学系

Recommended Citation:
李晓光,胥婕,刘宝明,等. 未经治疗的慢性乙型肝炎患者乙型肝炎病毒耐药变异、基因型和血清型研究[J]. 中华肝脏病杂志,2010,18(2):92-95.
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