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IR@PKUHSC  > 北京大学第三临床医学院  > 感染疾病科  > 期刊论文
学科主题: 传染病学
题名:
非诺特罗对内毒素诱导的 MyD88表达发挥抑制作用与抗炎效应相关
其他题名: Inhibition of LPS-induced expression of myeloid differentiation factor 88 by fenoterol is associated with its anti-inflammatory effect
作者: 王伟; 胥婕; 贺蓓
关键词: 非诺精罗 ; 单核细胞 ; LPS ; 抗炎 ; MyD88 ; Fenoterol ; Monocyte ; LPS ; Anti-inflammation ; Myeloid differentiation factor 88
刊名: 实用医学杂志
发表日期: 2014
DOI: 10.3969/j.issn.1006-5725.2014.18.008
期: 18, 页:2890-2893
收录类别: 中国科技核心期刊 ; 中文核心期刊
文章类型: Journal Article
摘要: 目的:探讨β2肾上腺素受体激动剂非诺特罗(fenoterol)在单核细胞上抑制内毒素(lipopolysaccharide,LPS)诱导炎症的分子机制。方法:选择 THP-1单核细胞系,ELISA(酶联免疫吸附剂测定)方法检测在有或无 fenoterol 预先干预下,LPS 刺激的 THP-1细胞上清中炎症因子肿瘤坏死因子-α(TNF-α)、单核细胞趋化蛋白-1(MCP-1)分泌及野生型和 MyD88-/-(髓样分化因子,myeloid differentiation factor 88)小鼠腹腔巨噬细胞上清中炎症因子 TNF-α、IL-1β(白介素-1β)分泌;western blot 方法检测有或无fenoterol 预先干预下,LPS 刺激的 MyD88表达。结果:fenoterol 可以显著抑制 THP-1细胞上 LPS 诱导的MyD88的表达及 TNF-α、MCP-1的分泌和小鼠巨噬细胞 TNF-α、IL-1β的分泌,MyD88-/-小鼠腹腔巨噬细胞对 LPS 的反应显著低于野生型小鼠腹腔巨噬细胞。结论:MyD88在 LPS 诱导的炎症中发挥重要作用, fenoterol 对 LPS 诱导的 MyD88表达发挥抑制作用与其抗炎效应相关。 Objective To explore the molecular mechanism of inhibition of LPS-induced inflammation by fenoterol, a β2 adrenoceptor agonist in monocyte. Methods Concentrations of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) and MCP-1 from cell supernatants from THP-1 cells and wild type or MyD88- / - mice peritoneal macrophages stimulated by LPS in the presence or absence of fenoterol were determined by use of an ELISA system. Expression of MyD88 (myeloid differentiation factor 88) stimulated by LPS in the presence or absence of fenoterol were determined by Western blot. Results Fenoterol inhibited LPS-induced activation of MyD88 and secretion of inflammatory cytokines (TNF-α, MCP-1, and IL-1β). The reaction of MyD88- / - mice peritoneal macrophages to LPS was much lower than that of the wild type mice peritoneal macrophages. Conclusions MyD88 plays an important role in inflammation induced by LPS. The inhibition of LPS-induced expression of MyD88 by fenoterol is associated with its anti-inflammatory effect.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/70327
Appears in Collections:北京大学第三临床医学院_感染疾病科_期刊论文

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作者单位: 呼吸科, 北京大学第三医院感染疾病科,100191

Recommended Citation:
王伟,胥婕,贺蓓. 非诺特罗对内毒素诱导的 MyD88表达发挥抑制作用与抗炎效应相关[J]. 实用医学杂志,2014(18):2890-2893.
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