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IR@PKUHSC  > 北京大学第三临床医学院  > 骨科  > 期刊论文
学科主题: 骨科学
题名:
p16INK4a蛋白在正常及骨关节炎关节软骨细胞中的表达及差异分析
其他题名: Expressions of p16INK4a in healthy and osteoarthritic human articular cartilage and difference analysis
作者: 周昊嵬; 娄思权; 程爱新; 张克
关键词: 骨性关节炎 ; 衰老 ; 基因,p16
刊名: 中国临床康复
发表日期: 2004
DOI: 10.3321/j.issn:1673-8225.2004.11.060
卷: 8, 期:11, 页:2148-2149
文章类型: Journal Article
摘要: 背景:在诸多老化和衰老的研究中,p16INK4a是研究较多的一种产物,然而其在与衰老密切相关的骨性关节炎中的研究较少.目的:观察p16INK4a在正常和骨性关节炎软骨细胞中的表达,讨论其在骨性关节炎病理过程中的作用.设计:以诊断为依据设立对照的实验研究.地点和对象:实验在北京大学第三医院骨科完成,对象为骨性关节炎软骨标本,取材于在北京大学第三医院骨科手术的膝骨关节炎患者.正常老年、中年及青年组软骨标本分别取自无骨性关节炎患者.干预:直接从软骨组织中提取蛋白和细胞的总RNA,分别进行Westernblot和RT-PCR分析,检测p16INK4a在年轻、中年、正常老年人及骨性关节炎患者软骨中的表达情况.对p16INK4a下游底物pRb的磷酸化状态也进行了蛋白表达的分析.主要观察指标:p16INK4a在蛋白水平及mRNA水平上的表达.结果:在关节软骨细胞中,p16INK4a的表达随着年龄的增长,其表达量也逐渐增多;相应地,p16INK4a的主要靶物质pRb的磷酸化明显减少,相反非磷酸化的pRb的含量则相对增高.与正常人相比,这些变化在骨性关节炎的软骨细胞中尤为显著.结论:p16INK4a/pRb途径可能在关节软骨细胞的老化及骨性关节炎的发病过程中具有一定的调节作用.p16INK4a表达的升高以及随后的pRb磷酸化状态的丢失可能参与了关节软骨细胞的老化及骨性关节炎的发生. BACKGROUND: Intensive studies of p16INK4a has been carried on in the research of aging, but its role in the pathogenesis of osteoarthritis(OA),which is closely related to aging, has not been thorouglly examined.OBJECTIVE: To determine the expression of p16INK4a in human articular chondrocytes for understanding its possible role in the pathogenesis of OA.DESIGN: A controlled experiment was conducted on the basis of clinical diagnosis.SETTING and PARTICIPANTS: All samples were obtained from the patients undergoing surgery in the Department of Orthopeadic Surgery, Third Hospital of Peking University. Osteoarthritic cartilage samples were from 26 senior patients undergoing total knee replacement. Also collected were the normal cartilage samples from 13 age-matched subjects, 17 young and 13 middle-ged subjects, who exhibited no clinical signs of OA.INTERVENTIONS: The total RNA was extracted from the chondrocytes of the OA patients and healthy young, middle-aged and senior subjects for determination of p16INK4a protein and mRNA expressions by means of Western blotting and reverse transcriptase-PCR(RT-PCR) respectively. Phosphorylation status of pRb, an established target of p16INK4a, was also examined by Western blotting.MAIN OUTCOME MEASURES: The expressions ofpl6INK4a protein and mRNA levels were measured.RESULTS: p16INK4a expression in the chondrocytes tended to increase with age, but no significant difference in its expressions was found between senior and middle-aged control subjects. OA patients, in contrast, had significantly increased p16INK4a expressions, and accordingly, loss of phosphorylated pRb and elevated levels of unphosphorylated pRb were observed in these patients.CONCLUSION: Alterations of pl6INK4a/pRb pathway may be involved in the aging of the chondrocytes and pathogenesis of OA, in which processes p16INK4 overexpression and the subsequent loss of phosphorylated pRb play an important role.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/70844
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 北京大学第三医院骨科,北京市,100083

Recommended Citation:
周昊嵬,娄思权,程爱新,等. p16INK4a蛋白在正常及骨关节炎关节软骨细胞中的表达及差异分析[J]. 中国临床康复,2004,8(11):2148-2149.
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