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学科主题: 呼吸内科学
题名:
内源性硫化氢对吸烟大鼠小气道纤维化的保护作用
其他题名: Hydrogen sulfide protects against smoke-induced small airway fibrosis in rats
作者: 廖程程; 陈亚红; 林帆; 孙云; 白宇; 齐永芬
关键词: 硫化氢 ; 慢性阻塞性肺疾病 ; 小气道纤维化 ; Hydrogen sulfide ; Chronic obstructive pulmonary disease ; Small airway fibrosis
刊名: 国际呼吸杂志
发表日期: 2014
DOI: 10.3760/cma.j.issn.1673-436X.2014.16.009
期: 16, 页:1228-1232
收录类别: 中国科技核心期刊
文章类型: Journal Article
摘要: 目的:探讨内源性硫化氢(H 2 S)对于吸烟诱导的 COPD 大鼠模型中小气道纤维化的影响。方法32只健康雄性 SD 大鼠随机分为正常组、吸烟组、吸烟+H 2 S 组和吸烟+炔丙基甘氨酸(PPG)组,采用被动烟雾吸入法,共吸烟4个月,从第3个月起,每日吸烟前给予大鼠腹腔注射生理盐水、硫氢化钠(NaHS)(14μmol/kg)或 PPG(37.5 mg/kg),取肺组织切片进行 HE 染色和天狼星红染色,观察肺组织病理形态改变,对小气道胶原沉积采用半定量分析,并进行小气道病理评分。Western blot 检测肺组织胱硫醚-γ-裂解酶(CGL)、Ⅰ型胶原蛋白表达水平。结果与正常组比较,吸烟组小气道病理评分明显升高(P <0.01),NaHS 干预后较吸烟组评分降低(P <0.05)。偏振光显微镜下观察天狼星红染色切片,吸烟组和吸烟+PPG 组较正常组小气道周围胶原沉积显著增多(P <0.01),吸烟+ H 2 S 组较吸烟组明显减少(P <0.05)。与正常组相比,吸烟组大鼠肺组织Ⅰ型胶原蛋白含量明显增多(P <0.05),NaHS干预后Ⅰ型胶原蛋白含量较单纯吸烟组明显降低(P <0.05)。结论内源性 H 2 S 参与吸烟诱导的COPD 大鼠小气道纤维化的病理生理过程,外源性给予 H 2 S 对小气道纤维化具有保护作用。 Objective To investigate the effect of hydrogen sulfide (H 2 S)in small airway fibrosis of rats with chronic obstructive pulmonary disease (COPD).Methods Thirty-two male Sprague-Dawley rats were randomly divided into four groups:control group,tobacco smoke group,smoke+H 2 S group,and smoke+PPG group.Drug intervention started at the third month of a total four-month smoke exposure. Rats were intraperitoneally injected with normal saline,sodium hydrosulfide (NaHS,14 μmol/kg),or PPG (37.5 mg/kg)before smoke every day.After four months,the histological change of lungs and the collagen deposition in airway were measured by HE staining and picrosiriu-red staining.The pathological score of small airway was calculated.The expressions of cystathionine γ-lyase and collagen Ⅰ were measured by Western blot.Results Compared with control group,the pathological score of small airway was significantly elevated in smoke group (P < 0.01 ),but decreased after NaHS intervention (P <0.05).Picrosiriu-red staining was detected under a polarided microscope.Compared with control group, the collagen deposition around the airway wall was increased in smoke group and smoke+PPG group (P <0.01).And the collagen deposition in smoke+H 2 S group was less than that in smoke group (P <0.05). Compared with control group,the expression of collagenⅠwas significant elevated in smoke group (P <0.05),but decreased after NaHS intervention (P <0.05).Conclusions Endogenous H 2 S plays a crucial role in the small airway fibrosis in smoke-induced COPD rats.Exogenously applied H 2 S could attenuate the process of airway fibrosis.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/71067
Appears in Collections:北京大学第三临床医学院_呼吸内科_期刊论文

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作者单位: 1.100191,北京大学第三医院呼吸科
2.100191,北京大学医学部分子心血管教育部重点实验室

Recommended Citation:
廖程程,陈亚红,林帆,等. 内源性硫化氢对吸烟大鼠小气道纤维化的保护作用[J]. 国际呼吸杂志,2014(16):1228-1232.
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