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学科主题: 呼吸内科学
题名:
哮喘豚鼠气道重塑与气道反应性的图像分析
其他题名: Image analysis of airway remodeling and responsiveness in asthmatic guinea pig
作者: 章晓初; 姚婉贞; 赵鸣武; 何其华; 陈月
关键词: 哮喘 ; 气道重塑 ; 气道反应性 ; 图像分析
刊名: 中华结核和呼吸杂志
发表日期: 2001
卷: 24, 期:2, 页:87-89
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的研究哮喘豚鼠气道重塑的机制及气道反应性的变化。方法实验分二组:对照组(20只)和哮喘组(20只),取双肺与不同刺激因素作用后作组织切片、HE染色,通过图像分析仪测定支气管内周长、管壁厚度、外周长,并计算平滑肌收缩百分比(PMS)。结果(1)哮喘组支气管壁厚度(WA/Pi)为(10.0±2.0)μm2/μm,支气管壁平滑肌厚度(平滑肌的面积/Pi)为(4.8±1.5)μm2/μm,对照组WA/Pi为(7.9±2.1)μm2/μm,平滑肌的面积/Pi为(3.1±2.0)μm2/μm,两组比较差异均有显著性(P<0.01),支气管壁平滑肌细胞核数量(N/Pi)哮喘组为(0.012 3±0.002 7)个/μm,对照组为(0.010±0.003)个/μm,两组比较差异有显著性(P<0.05)。(2)哮喘组气道平滑肌对腺苷的反应性(以PMS表示)为0.34±0.07,对照组为0.29±0.08,两组比较差异有显著性(P<0.05),联用氨茶碱和腺苷后,哮喘组PMS为0.26±0.07,与单用腺苷组(0.34±0.07)比较差异有显著性(P<0.01)。(3)在Ach作用下哮喘组的PMS为0.24±0.04,对照组为0.19±0.06,两组比较差异有显著性(P<0.05),联用肝素和Ach后,哮喘组PMS为0.20±0.04,与单用Ach组(0.24±0.04)比较差异有显著性(P<0.05)。结论哮喘气道平滑肌增生是气道重塑的主要原因,氨茶碱和肝素可以抑制气道对腺苷和乙酰胆碱的反应性。 Objective To observe the mechanism of airway remodeling and changes of airway responsiveness in guinea pig model of asthma. Methods 40 guinea pigs were randomly divided into two groups: control (20)and asthmatic group (20). After incubating with different stimulus, bilateral lung tissue section were stained with HE. Using image analysis system to measure the airway internal perimeter, wall area, external perimeter, etc. and calculate percentage of muscle shortening (PMS) according to formula. Results (1) The airway wall thickness (WA/Pi) in asthmatic group and control group were (10.0±2.0 )and (7.9±2.1) μm2/μm, respectively. The bronchial smooth muscle thickness in asthmatic group and control group were (4.8±1.5 )and (3.1±2.0) μm2/μm, respectively. Both were statistically significant (P<0.01). The number of bronchial smooth muscle nucleus in asthmatic group (0.012 3±0.002 7/μm) was higher compared with control (0.010±0.003/μm) (P<0.05). (2) Responsiveness of airway smooth muscle to adenosine (represented by PMS) in asthmatic group and control were 0.34±0.07 and 0.29±0.08,respectively (P<0.05). When combined with aminophyline, PMS in asthmatic group was 0.26±0.07. There was statistically significant difference when compared with adenosine alone (0.34±0.07) (P<0.01). (3) PMS to acetylcholine in asthmatic group and control were 0.24±0.04 and 0.19±0.06, respectively (P<0.05). When combined with heparin, PMS in asthmatic group was 0.20±0.04. There was statistically significant difference when compared with acetylcholine alone (0.24±0.04 ) (P<0.05).Conclusions The main reason of airway remodeling in asthma is due to bronchial smooth muscle hyperplasia. Aminophylline and heparin may inhibit the responsiveness of airway to adenosine and acetylcholine respectively.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/71278
Appears in Collections:北京大学第三临床医学院_呼吸内科_期刊论文

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作者单位: 1.北京大学第三医院呼吸科
2.北京大学医学部医药分析中心

Recommended Citation:
章晓初,姚婉贞,赵鸣武,等. 哮喘豚鼠气道重塑与气道反应性的图像分析[J]. 中华结核和呼吸杂志,2001,24(2):87-89.
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