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大鼠慢性阻塞性肺疾病模型气道和肺组织M受体水平及溴化异丙托品的影响
其他题名Effect of ipratropium bromide on airway and pulmonary muscarinic receptors in a rat model of chronic obstructive pulmonary disease
姚婉贞; 王国扬; 朱红; 孙永昌; 赵鸣武
关键词受体毒蕈碱慢性阻塞性肺疾病动物模型 receptor muscarinic chronic obstructive pulmonary disease animal model
刊名中华医学杂志(英文版)
2001
DOI10.3760/j.issn:0366-6999.2001.01.018
114期:1页:80-83
文章类型Journal Article
摘要目的观察大鼠慢性阻塞性肺疾病(COPD)模型气道和肺组织M受体水平,以及吸入溴化异丙托品对M受体的影响及其规律。 方法通过长期吸入高浓度SO2气体的方法(250?ppm, 5?h/d, 5?d/wk, 共7?wk)建立COPD模型, 大鼠在密闭箱内吸入雾化的0.025%溴化异丙托品溶液10?ml(2次/d, 20?min/次),采用放射配基结合法,分别测定正常大鼠、吸入和未吸入异丙托品大鼠气道和肺组织的M受体。 结果支气管肺病理学及肺功能检查显示,大鼠长期吸入高浓度SO2气体可引起与人类COPD相似的病理生理改变。COPD大鼠气道和肺组织M受体的密度(0.038±0.011?pmol/mg蛋白质)及亲和力(Kd, 23±11?pmol/L)与正常大鼠相比(分别为0.030±0.008,29±19)无明显变化(P>0.05)。吸入异丙托品30天后,气道和肺组织M受体密度显著升高(P<0.05),停药6天后,M受体的密度恢复正常。 结论长期吸入高浓度SO2可制备与人类COPD病理生理改变相似的大鼠模型。COPD大鼠气道和肺组织M受体的数量及功能无异常改变,但长期应用异丙托品可使M受体上调。 Objective To observe the level of muscarinic receptors in airway and lung tissues, and the effect of inhaled ipratropium bromide on these receptors in a rat model of chronic obstructive pulmonary disease (COPD). Methods This model was developed by exposure of rats to 250?ppm SO2 gas, 5?h/d, 5?d/wk, for a period of 7?wk. The COPD rats inhaled 0.025% aerosolized iratropium bromide for 20?min, 2 times daily, in an airtight chamber. Muscarinic receptors in airway and lung tissues of normal rats, ipratropium bromide-treated COPD rats and the recovering COPD rats were measured by the radio-ligand binding assay. Results Airway/lung pathology and pulmonary function tests showed that chronic SO2 exposure caused pathophysiologic changes similar to those observed in human COPD. The density (0.038±0.011, pmol/mg protein) and affinity (Kd, 23±11?pmol/L) of muscarinic receptors in airway and lung tissues of COPD rats were not changed compared with those of normal control rats (0.030±0.008 and 29±19, respectively, P>0.05). Densities of the muscarinic receptors were not changed after inhalation of ipratropium bromide for 5 days, but increased significantly after inhalation for 30 days, as compared with those of the untreated COPD rats. The muscarinic receptors returned the normal levels at day 6 after cessation of ipratropium bromide treatment. There were no differences among different groups of rats in equilibrium dissociation constants (Kd). Conclusion A rat model of COPD with pathophysiologic changes similar to the human counterpart was developed using chronic SO2 exposure. There was no significant change in the number and function of muscarinic receptors in airway and lung tissues of the COPD rats, but upregulation of the muscarinic receptors was observed after long-term inhalation of ipratropium bromide.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/71282
专题北京大学第三临床医学院_呼吸科
作者单位北京大学第三医院呼吸科北京 100083
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GB/T 7714
姚婉贞,王国扬,朱红,等. 大鼠慢性阻塞性肺疾病模型气道和肺组织M受体水平及溴化异丙托品的影响[J]. 中华医学杂志(英文版),2001,114(1):80-83.
APA 姚婉贞,王国扬,朱红,孙永昌,&赵鸣武.(2001).大鼠慢性阻塞性肺疾病模型气道和肺组织M受体水平及溴化异丙托品的影响.中华医学杂志(英文版),114(1),80-83.
MLA 姚婉贞,et al."大鼠慢性阻塞性肺疾病模型气道和肺组织M受体水平及溴化异丙托品的影响".中华医学杂志(英文版) 114.1(2001):80-83.
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