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学科主题临床检验诊断学
生长激素释放肽促进胰岛β细胞增殖的实验研究
其他题名Ghrelin promotes proliferation and Inhibits apoptosis of pancreatic β cells
刘荣凤; 张捷; 胡晓舟; 王小林
关键词胰岛 细胞增殖 肽类激素 Islets of langerhans Cell proliferation Peptide hormones
刊名中华检验医学杂志
2008
DOI10.3321/j.issn:1009-9158.2008.09.019
31期:9页:1039-1042
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的 研究生长激素释放肽(ghrelin)对胰岛β细胞(NIT-1)增殖的影响,并初步探讨其作用机制.方法 采用不同浓度的ghrelin在不同时间内对NIT-1细胞进行刺激,以噻唑蓝(MTT)法观察ghrelin对NIT-1细胞增殖的影响;采用流式细胞术检测ghrelin对细胞周期的影响;采用免疫印迹法(Western blot)分析细胞外信号调节激酶(ERK1/2)表达水平和ERK1/2磷酸化水平.结果 随着ghrelin浓度的增加和作用时间的延长,发现其有促进NIT-1细胞增殖的作用.ghrelin作用于NIT-1细胞48 h后,改变细胞S期所占比例,ghrelin浓度为0、10-9、10-8、10-7 mol/L时,对应的S期细胞所占比例分别为(34.5±6.5)%、(42.1±7.4)%、(50.6±5.8)%、(71.4±9.4)%.ghrelin处理NIT-1细胞后,ERK1/2出现磷酸化,且磷酸化水平与ghrelin浓度呈剂量-效应关系.结论 ghrelin通过激活丝裂原活化蛋白激酶(MAPK)/ERK1/2途径,影响细胞周期,促进胰岛β细胞增殖. Objective To investigate the role of ghrelin promoting proliferation of pancreatic β cells and the mechanism of it. Methods Mouse pancreatic β cells(NIT-1)were treated with different concentrations of ghrelin.NIT-1 cells proliferation was measured by MTT incorporation assay,and the cell cycle was measured by Flow Cytometry,and the expression of ERK1/2 and the level of ERK1/2 phosphorylation were determined by Western blot assay.Results With the increase of concentration and the time of treatment,ghrelin promotes cell survival of pancreatic β cells.The S-phase portion was changed after treatment of ghrelin on NIT-1 for 48 hours.The S-phase percentage in the groups where ghrelin concention change from 0 tO 10-9,10-8,10-7 mol/L were(34.5±6.5)%,(42.1±7.4)%,(50.6±5.8)%,(71.4±9.4)%,respectively.Ghrelin also induces phesphorilation of ERK1/2 in NIT-1 cell,and a doseeffect relationship was demonstrated.Conclusion Ghrelin could promote proliferation of pancreatic β cells through activating the MAPK/ERK1/2 signaling pathway and changing the cell cycle.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/71557
Collection北京大学第三临床医学院_检验科
作者单位北京大学第三医院检验科,100083
Recommended Citation
GB/T 7714
刘荣凤,张捷,胡晓舟,等. 生长激素释放肽促进胰岛β细胞增殖的实验研究[J]. 中华检验医学杂志,2008,31(9):1039-1042.
APA 刘荣凤,张捷,胡晓舟,&王小林.(2008).生长激素释放肽促进胰岛β细胞增殖的实验研究.中华检验医学杂志,31(9),1039-1042.
MLA 刘荣凤,et al."生长激素释放肽促进胰岛β细胞增殖的实验研究".中华检验医学杂志 31.9(2008):1039-1042.
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