北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 检验科  > 期刊论文
学科主题: 临床检验诊断学
题名:
流式细胞仪性能评价方法的建立
其他题名: Establishment of evaluation methods for the performance of flow cytometer
作者: 王小林; 李昂; 杨硕
关键词: 流式细胞仪 ; 性能评价 ; BriCyte E6 ; flow cytometry ; performance evaluation ; BriCyte E6
刊名: 国际检验医学杂志
发表日期: 2015
DOI: 10.3969/j.issn.1673-4130.2015.10.020
期: 10, 页:1366-1367,1369
文章类型: Journal Article
摘要: 目的:建立流式细胞仪性能评价方法。方法基于《YY/T0588‐2005流式细胞仪》行业标准,利用BriCyte E6流式细胞仪,建立适用于流式细胞仪性能评价的测试方案,包括荧光灵敏度、荧光线性、前向角散射光检测灵敏度、仪器分辨率、前向角散射光和侧向角散射光分辨率、倍体分析线性、携带污染率、表面标志物检测准确性、表面标志物检测重复性、仪器稳定性等。结果该流式细胞仪各项性能指标均满足行业标准规定的技术要求。结论上述性能指标测试方法可靠,能够对流式细胞仪性能进行全面评价。该方法对于流式细胞仪性能验证有一定的指导意义。 Objective To Establish evaluation methods for the performance of flow cytometer .Methods Referring to the indus‐try standard YY/T0588‐2005 Flow Cytometry ,evaluating methods for the performance of BriCyte E6 flow cytometry was estab‐lished ,such as fluorescence sensitivity ,fluorescence linearity ,forward scatter sensitivity ,instrument resolution ,forward scatter/side scattering resolution ,DNA content linearity ,carry‐over rate ,accuracy of the cell surface marker ,reproducibility of the cell surface marker and instrument stability .Results The performance of BriCyte E6 met the requirements of industry standard .Conclusion The evaluation methods for the performance parameters could be reliable and could be used for the performance evaluation of flow cytometer .
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/71630
Appears in Collections:北京大学第三临床医学院_检验科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 北京大学第三医院检验科,北京,100191

Recommended Citation:
王小林,李昂,杨硕. 流式细胞仪性能评价方法的建立[J]. 国际检验医学杂志,2015(10):1366-1367,1369.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[王小林]'s Articles
[李昂]'s Articles
[杨硕]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[王小林]‘s Articles
[李昂]‘s Articles
[杨硕]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace