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IR@PKUHSC  > 北京大学第三临床医学院  > 介入血管外科  > 期刊论文
学科主题: 血管外科学
题名:
洛伐他汀对人血管内皮细胞双向调节作用的实验研究
其他题名: An in-vitro study of Lovastatin's biphasic effects on proliferation and adhesion of human umbilical vein endothelial cells
作者: 翟国钧; 董国祥; 赵军; 姚伟娟
关键词: 内皮细胞 ; 洛伐他汀 ; 细胞增殖 ; 细胞黏附 ; 脐静脉 ; Endothelial cells ; Lovastatin ; Cell proliferation ; Cell adhesion ; Umbilical veins
刊名: 中华普通外科杂志
发表日期: 2010
DOI: 10.3760/cma.j.issn.1007-631X.2010.08.021
卷: 25, 期:8, 页:665-667
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨洛伐他汀对人血管内皮细胞的调节作用及其浓度相关性.方法 配制含不同浓度洛伐他汀的培养基(0.001、0.01、0.1、1、10μmol/L),分别于96孔板中进行人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)培养,在培养24、72、120 h后以噻唑蓝比色法(MTT)法进行细胞活性及增殖情况评估.将HUVECs接种于6孔板中,分别加入含上述不同浓度洛伐他汀的培养基,培养24 h后,消化细胞,计数,按照2×104/ml每孔接种在24孔板中,30 min后,洗掉尚未黏附的细胞,将余下的细胞每孔随机选一个视野,在200×视野中计量黏附细胞数,比较各组细胞的黏附情况. 结果培养24 h,0.01、0.1μmol/L的洛伐他汀对HUVECs存在明显的促进增殖作用;培养72 h,1μmol/L的洛伐他汀对HUVECs存在明显的促进增殖作用;培养120 h,0.001、0.01、0.1、1 μmol/L的洛伐他汀与对照组相比HUVECs增殖活性差异无统计学意义,10μmol/L的洛伐他汀表现出明显的增殖抑制作用.洛伐他汀与HUVECs作用24 h后,0.1、1μmol/L的洛伐他汀表现出明显的促进HUVECs黏附作用.结论 洛伐他汀对人血管内皮细胞存在双向调节作用,与浓度相关.体外浓度0.1、1μmol/L时可以表现出明显的促进增殖及黏附作用,浓度达10 μmol/L时则表现出抑制作用. Objective To investigate Lovastatin's effects on proliferation and adhesion of human umbilical vein endothelial cells (HUVECs). Methods Culture medium with different concentration of Lovastatin(0.001、0.01、0.1、1.0、10μmol/L) was prepared, HUVECs was cultured in 96 well-plate with the different medium. AT the point of 24,72 and 120 h, the cell's activity and quantity was assessed by MTT. HUVECs was cultured with Lovastatin in 6 well-plate for 24 hours, then collected the cells by trypsin digestion. The cells were seeded in 24 well-plate with 2×104/ml and adhering for 30 mins. Then counting the adhered cells in different wells. Results At 24 h, Lovastatin (0.01、 0.1 μmol/L ) promoted proliferation of HUVECs ( P < 0.05 ); at 72 h, Lovastatin ( 1.0μmol/L) was positive accelerating cell growth(P< 0.05 ). While Lovastatin ( 10μmol/L) inhibited the proliferation significantly ( P <0.05 ) at 120 h. As HUVECs was cultured with Lovastatin for 24 hours, Lovastatin (0.1、1μmol/L) inhanced the adhesion capability of HUVECs significantly( P< 0.05 ). Conclusion Lovastatin had biphasic effects on proliferation and adhesion of HUVECs dependent on the concentration. Lovastatin (0.1、1.0 μmol/L) could promote the proliferation and adhesion, while at higher concentration ( 10.0μmol/L) it inhibits cell proliferation and adhesion.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/71735
Appears in Collections:北京大学第三临床医学院_介入血管外科_期刊论文

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作者单位: 1.北京大学第三医院介入血管外科,100191
2.北京大学医学部生理系

Recommended Citation:
翟国钧,董国祥,赵军,等. 洛伐他汀对人血管内皮细胞双向调节作用的实验研究[J]. 中华普通外科杂志,2010,25(8):665-667.
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