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学科主题: 康复医学
题名:
海风藤对活化的新生大鼠小胶质细胞的影响作用
其他题名: Effects of Piper Kadsura Ohwi on natal rat microglial activation
作者: 邢华医; 马艳; 马学强; 郑梅梅; 杜怡峰
关键词: 小胶质细胞 ; 淀粉样蛋白寡聚体 ; 海风藤提取物 ; 阿尔茨海默病 ; Microglia ; Oligomeric Aβ ; Piper Kadsura Ohwi Extracts ; Alzheimer' ; s Disease
刊名: 中华行为医学与脑科学杂志
发表日期: 2011
DOI: 10.3760/cma.j.issn.1674-6554.2011.09.004
卷: 20, 期:9, 页:778-780
收录类别: 中国科技核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 研究海风藤提取物对纤丝状和寡聚体β淀粉样蛋白(β-amyloid,Aβ)诱导的活化新生大鼠小胶质细胞可能的影响.方法 原代培养新生大鼠小胶质细胞,分别用纤丝状Aβ25-35、寡聚体Aβ25-35、纤丝状Aβ25-35+海风藤提取物、寡聚体Aβ25-35+海风藤提取物干预小胶质细胞,并设立空白对照组.干预48h后,以CD68作为小胶质细胞活化的特异性抗原标志,利用免疫细胞化学染色方法,对小胶质细胞的活化率进行检测,并比较不同处理组间的差异.结果 四个实验组的小胶质细胞CD68表达阳性率均高于空白对照组(5.1%,P<0.05).纤丝状Aβ+海风藤提取物组CD68表达阳性率(52.1%)低于纤丝状Aβ组(60.8%,P<0.05).寡聚体Aβ组(71.2%)和寡聚体Aβ+海风藤提取物组(67.0%)的CD68表达阳性率无明显差异(P=0.101).结论 纤丝状和寡聚体Aβ均能激活小胶质细胞;海风藤提取物能够抑制纤丝状Aβ对小胶质细胞的激活作用,对寡聚体Aβ的激活作用无明显影响. Objective To compare the activation of microglia in vitro induced by oligomeric and fibrillar Aβ,and research the effects of Piper Kadsura Ohwi extracts on the microglial activation.Methods Microglia were divided randomly into 4 test groups,intervened by fibrillar Aβ25-35,fibrillar Aβ25-35 + Piper Kadsura Ohwi extracts,oligomeric Aβ25-35 and oligomeric Aβ25-35 + Piper Kadsura Ohwi extracts respectively.Also a blank contrast group was set without any intervention.48 hours later,activation of microglia was tested by immunocytochemistry,using the CD68 molecule as a specific marker of microglial activation and the incidences of active microglia in different groups were compared.Results Each of the 4 test groups had a higher positive incidence of CD68 expression than that of the blank contrast (5.1% ) (P < 0.05 ) ; positive incidence of fibrillar Aβ + Piper Kadsura Ohwi extracts group (52.1%) was lower than that of the fibrillar Aβ group (60.8%) (P<0.05) ; positive incidence of oligomeric Aβ + Piper Kadsura Ohwi extracts group (67.0%) was not significantly different with that of the oligomeric Aβ group (71.2%),P=0.101.Conclusion Both fibrillar and oligomeric Aβ has the ability to activate the silent microglia.Piper Kadsura Ohwi extracts could inhibit the activation of microglia induced by fibrillar Aβ25-35,but didn't show significant effects on the activation induced by oligomeric Aβ25-35.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/71869
Appears in Collections:北京大学第三临床医学院_康复医学科_期刊论文

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作者单位: 1.北京大学第三医院康复医学中心
2.河北省保定市人民医院
3.山东电力中心医院神经内科
4.北京天坛医院神经内科
5.山东大学附属省立医院神经内科,济南,250021

Recommended Citation:
邢华医,马艳,马学强,等. 海风藤对活化的新生大鼠小胶质细胞的影响作用[J]. 中华行为医学与脑科学杂志,2011,20(9):778-780.
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