北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 麻醉科  > 期刊论文
学科主题: 麻醉学
题名:
不同剂量脂多糖在不同作用时间下诱导小鼠急性肺损伤的效果评价
其他题名: An evaluation of effects on induction of acute lung injury in mice by different doses of lipopolysaccharide and different durations
作者: 陶一帆; 田方敏; 郭向阳; 朱怀球; 朱曦
关键词: 脂多糖 ; 肺损伤,急性 ; 炎症因子 ; Lipopolysaccharide ; Acute lung injury ; Inflammatory factor
刊名: 中国中西医结合急救杂志
发表日期: 2015
DOI: 10.3969/j.issn.1008-9691.2015.02.008
期: 2, 页:142-146
收录类别: 中国科技核心期刊 ; 中文核心期刊
文章类型: Journal Article
摘要: 目的:观察脂多糖(LPS)诱导急性肺损伤(ALI)小鼠的炎症因子变化,探讨不同剂量LPS在ALI发生发展过程中不同时间点对肺损伤的影响。方法将210只C57BL/6小鼠按气管内滴注LPS剂量分为2.5、5.0、7.5、10.0 mg/kg组,采用气管内滴注LPS的方法复制ALI模型;并设正常对照组、生理盐水对照组,每组10只。LPS损伤后1、2、4、8 h观察小鼠的肺组织湿/干质量(W/D)比值、肺损伤评分及肺组织病理学的变化,同时检测血清和支气管肺泡灌洗液(BALF)中去甲肾上腺素(NE)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和蛋白含量。结果①LPS所致肺损伤呈剂量依赖性增加和时间依赖性增加。②随LPS剂量增加和作用时间延长,LPS组肺组织W/D比值、肺损伤评分增加,血清和BALF中NE、TNF-α、IL-6及BALF中蛋白含量均显著增加,LPS 5.0 mg/kg组作用4 h是出现急性呼吸窘迫综合征(ARDS)显著特征的剂量和时间点〔肺W/D比值:4.97±0.41,肺损伤评分(分):5.60±1.52;血清NE(ng/L):379.99±27.65, TNF-α(ng/L):159.15±20.62,IL-6(ng/L):177.15±29.13;BALF 中 NE(mg/kg):105.85±13.66,TNF-α(mg/kg):227.22±48.01,IL-6(mg/kg):251.55±54.08,总蛋白含量(g/L):1.59±0.37〕,但上述指标的变化以LPS 10.0 mg/kg组作用后8 h较LPS 7.5、5.0、2.5 mg/kg组相同时间点更显著〔肺组织W/D比值:5.10±0.18比5.01±0.43、5.01±0.19、4.91±0.30,肺损伤评分(分):9.20±1.48比8.00±1.00、6.00±1.22、4.40±0.89;血清NE(ng/L):447.43±34.63比419.23±30.62、391.16±54.91、372.59±51.52,TNF-α(ng/L):205.99±31.31比181.01±25.11、161.01±13.98、138.83±28.95,IL-6(ng/L):233.76±34.84比206.21±26.68、186.58±26.54、156.99±28.83;BALF 中 NE(mg/kg):190.82±41.75比153.30±35.42、122.64±25.15、80.23±13.69,TNF-α(mg/kg):305.24±72.99比292.77±38.07、249.60±35.20、193.63±10.83,IL-6(mg/kg):354.81±67.79比303.02±54.24、272.43±32.34、197.64±12.35,BALF总蛋白含量(g/L):2.31±0.30比2.02±0.26、1.62±0.19、1.10±0.24,P<0.05或P<0.01〕。结论 LPS诱导的小鼠ALI程度与剂量和时间呈正相关。LPS 5.0 mg/kg作用4 h是出现ARDS显著特征的剂量和时间点。 Objective To observe the changes of inflammatory factors in acute lung injury (ALI) in mice induced by lipopolysaccharide (LPS), and to explore the influence of different doses of LPS on ALI onset and progress at different time points. Methods Intratracheally, LPS at the dosages of 2.5, 5.0, 7.5 and 10.0 mg/kg were administered to a total of 210 C57BL/6 mice, and according to the difference in dosage, they were divided into four groups. The ALI model was replicated by intratracheally dropping of LPS. And a normal control group and a normal saline control group were established (each, n=10). The changes of index of pathological lung tissue and lung tissue wet/dry (W/D) ratio were observed at 1, 2, 4, and 8 hours after injury, and simultaneously, the levels of norepinephrine (NE), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and protein in serum and bronchoalveolar lavage fluid (BALF) were detected. Results ①The degree of lung injury induced by LPS was dose-and time-dependent.②With the increase of LPS dosage and prolongation of time, in LPS group, the lung W/D ratio and the index of pathological lung tissue were increased;additionally, the levels of NE, TNF-α, IL-6 and protein in serum or BALF were also significantly increased. The critical occurrence point of acute respiratory distress syndrome (ARDS) with specific characteristics was at 5.0 mg/kg of LPS acting for 4 hours [lung W/D ratio: 4.97±0.41, index of pathological changes of lung tissue (score): 5.60±1.52; serum NE (ng/L): 379.99±27.65, TNF-α (ng/L): 159.15±20.62, IL-6 (ng/L): 177.15±29.13;BALF NE (mg/kg):105.85±13.66, TNF-α(mg/kg):227.22±48.01, IL-6 (mg/kg):251.55±54.08, total protein (g/L):1.59±0.37]. The injury induced by LPS acting for 8 hours in the dosage group 10.0 mg/kg was the most significant in comparisons with other groups of dosages at the same time points [lung W/D ratio:5.10±0.18 vs. 5.01±0.43, 5.01±0.19, 4.91±0.30; index of pathological changes of lung tissue (score): 9.20±1.48 vs. 8.00±1.00, 6.00±1.22, 4.40±0.89;serum NE (ng/L): 447.43±34.63 vs. 419.23±30.62, 391.16±54.91, 372.59±51.52; TNF-α(ng/L): 205.99±31.31 vs. 181.01±25.11, 161.01±13.98, 138.83±28.95; IL-6 (ng/L): 233.76±34.84 vs. 206.21±26.68, 186.58±26.54, 156.99±28.83;BALF NE (mg/kg):190.82±41.75 vs. 153.30±35.42, 122.64±25.15, 80.23±13.69;TNF-α(mg/kg):305.24±72.99 vs. 292.77±38.07, 249.60±35.20, 193.63±10.83; IL-6 (mg/kg): 354.81±67.79 vs. 303.02±54.24, 272.43±32.34, 197.64±12.35;total protein (g/L):2.31±0.30 vs. 2.02±0.26, 1.62±0.19, 1.10±0.24, P<0.05 or P<0.01]. Conclusions The severity of ALI induced by LPS in mice was positively correlated to LPS dosage and duration of its action. After administration of LPS 5 mg/kg for 4 hours, remarkable characteristic manifestations of ARDS occur in mice, reaching the critical point.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/72187
Appears in Collections:北京大学第三临床医学院_麻醉科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.北京大学第三医院 麻醉科,北京,100191
2.北京大学工学院,北京,100871
3.北京大学第三医院 危重医学科,北京,100191

Recommended Citation:
陶一帆,田方敏,郭向阳,等. 不同剂量脂多糖在不同作用时间下诱导小鼠急性肺损伤的效果评价[J]. 中国中西医结合急救杂志,2015(2):142-146.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[陶一帆]'s Articles
[田方敏]'s Articles
[郭向阳]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[陶一帆]‘s Articles
[田方敏]‘s Articles
[郭向阳]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace