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利多卡因后处理减轻大鼠肺缺血再灌注损伤
其他题名Alleviative effects of lidocaine postconditioning on pulmonary ischemia-reperfusion injury of rats
徐懋; 高峰; 郭向阳
关键词缺血再灌注 利多卡因 后处理 ischemia reperfusion lidocaine postconditioning pulmonary
刊名基础医学与临床
2010
30期:1页:24-27
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的 探讨利多卡因后处理对肺缺血再灌注损伤的保护作用.方法 72只大鼠随机分为4组,每组18只:假手术组、I-R组(ischemia reperfusion,I-R)、缺血后处理组(ischemic postconditioning,IPC)和利多卡因后处理组.采用夹闭左肺门45 min、然后复灌2 h的方法建立在体肺I-R模型.利多卡因后处理组再灌注即刻泵入利多卡因4 mg/kg,而后4 mg/(kg·h)持续再灌注2 h.测定PaO_2,检测肺组织W/D、MDA和血清TNF-α浓度,并对肺组织进行组织形态学检查.结果 再灌注后利多卡因组的PaO_2显著高于I-R组(P<0.05);再灌注2 h后利多卡因组的MDA[(7.03±1.17)μmol/L]明显低于I-R组[(8.77±1.42)μmol/L](P<0.05);再灌注2 h后利多卡因组TNF-α浓度[(1.69±O.34)μg/L]低于I-R组[(2.52±0.54)μg/L](P<0.05).肺组织形态学检查发现利多卡因组的肺水肿和中性粒细胞积聚较I-R组明显减轻.结论 利多卡因后处理可以减轻过氧化损伤和TNF-α的表达,减轻肺水肿,具有肺保护效应. Objective To investigate the alleviative effects of lidocaine postconditioning on pulmonary injury following ischemia reperfusion. Methods Seventy-two adult SD rats were randomized to 4 groups; sham group, ischemia-reperfusion (I-R) group, ischemic postconditioning(IPC) group and lidocaine postconditioning group. The pulmonary ischemia-reperfusion model was established by occlusion of the left hilum of lung for 45 min and the reperfusion was taken by removing the clamp for 2 h. At the moment of reperfusion, lidocaine 4 mg/kg was injected as a priming dose following a continuous rate of 4 mg/(kg · h). PaO_2, TNF-α, W/D of left lung, the level of MDA of left lung tissue were measured. At the end of reperfusion left lung was removed for microscopy. Results After reperfusion PaO_2 of lidocaine group was much higher than that of I-R group (P<0.05). Lidocaine postconditioning induced a significant decrease in the level of MDA of lung tissue[(7. 03±1.17) μmol/L] compared with ischemia reperfusion group [(8.77±1.42) μmol/L] (P<0.05). Lidocaine postconditioning resulted in a lower level of TNF-α [(1. 69±0.34) μg/L] than that of I-R group [(2. 52±0. 54) μg/L] (P < 0. 05). Microscopic examination showed that lidocaine postconditioning could decrease the level of edema of left lung and accumulation of neutro-phils. Conclusion Lidocaine postconditioning exerts a protective effect on pulmonary ischemia-reperfusion injury administered in the beginning of reperfusion. The effect may be explained by to the antioxidant effect and the suppression of expression of TNF-α.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/72311
专题北京大学第三临床医学院_麻醉科
作者单位1.北京大学第三医院麻醉科,北京,100191
2.华中科技大学同济医学院附属同济医院,麻醉学教研室,湖北武汉,430030
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徐懋,高峰,郭向阳. 利多卡因后处理减轻大鼠肺缺血再灌注损伤[J]. 基础医学与临床,2010,30(1):24-27.
APA 徐懋,高峰,&郭向阳.(2010).利多卡因后处理减轻大鼠肺缺血再灌注损伤.基础医学与临床,30(1),24-27.
MLA 徐懋,et al."利多卡因后处理减轻大鼠肺缺血再灌注损伤".基础医学与临床 30.1(2010):24-27.
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