北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 泌尿外科  > 期刊论文
学科主题: 泌尿外科学
题名:
间苯三酚对大鼠肾缺血再灌注损伤的保护作用
其他题名: Protective effect of phloroglucinol on renal ischemia and reperfusion injury
作者: 李刚; 张洪宪; 王云鹏; 张径; 洪锴; 田晓军; 马潞林
关键词: 间苯三酚 ; ; 再灌注损伤 ; 氧化性应激 ; 细胞凋亡 ; Phloroglucinol ; Kidney ; Reperfusion injury ; Oxidative stress ; Apoptosis
刊名: 北京大学学报(医学版)
发表日期: 2015
DOI: 10.3969/j.issn.1671-167X.2015.05.003
期: 5, 页:743-748
文章类型: Journal Article
摘要: 目的:研究间苯三酚对大鼠肾缺血再灌注损伤( ischemia reperfusion injury , IRI)的保护作用及机制。方法:将雄性Wistar大鼠48只平均分为3组( n=16):假手术组( Sham组)切除大鼠右肾后,左肾动脉进行同等分离,但不予夹闭;对照组即肾缺血再灌注组( ischemia reperfusion , I/R组),腹腔注射等量的生理盐水,15 min后切除大鼠右肾,无创动脉夹夹闭左肾动脉45 min;实验组即缺血再灌注间苯三酚预处理组(phloroglucinol,PG组),腹腔注射间苯三酚注射液(30 mg/kg),15 min后切除大鼠右肾,无创动脉夹夹闭左肾动脉45 min。每组动物再均分为两个亚组(n′=8),分别于再灌注后6和24 h将大鼠处死。处死前经下腔静脉取血,检测血清肌酐(secrum creatinine, SCr)、尿素氮(blood urine nitrogen, BUN);将肾于冠状位切成两半,一半组织制作成组织匀浆,取组织上清液检测丙二醛(malondialdehyde, MDA)、过氧化氢酶(catalase, CAT)、超氧化物歧化酶(superoxide dismutase, SOD)及谷胱甘肽过氧化物酶( glutathione peroxidase , GSH-Px);另一半组织行石蜡包埋,切片进行病理组织学观察,再灌注后24 h的大鼠肾组织行核转录因子-kapa B(nuclear factor-kapa B, NF-κB)及Caspase-3免疫组织化学检测。结果:再灌注后6 h,I/R组大鼠血清SCr和BUN分别为(103.9±10.4)μmol/L和(15.2±1.0) mmol/L,PG预处理的SCr及BUN分别为(81.8±13.4)μmol/L和(11.5±1.2) mmol/L;再灌注后24 h,I/R组大鼠血清SCr和BUN分别为(154.9±12.1)μmol/L和(28.1±1.4) mmol/L,PG预处理的SCr及BUN分别为(103.8±5.9)μmol/L和(16.0±1.0) mmol/L;PG预处理组较I/R组明显改善肾功能(P<0.05);苏木素-伊红染色病理图片可见肾小管损伤较I/R组明显减轻(P<0.05);经PG处理后大鼠肾组织内MDA含量较I/R组低(P<0.05),SOD及CAT含量较I/R组高(P<0.05),GSH-Px含量较I/R组高(P<0.05)。再灌注后24 h,经间苯三酚处理的肾组织内核因子-kapa B在细胞核内表达的水平明显降低,活化的Caspase-3亦较I/R组有所减少。结论:间苯三酚通过减轻氧化应激和炎性损伤,抑制细胞凋亡,有效改善了大鼠肾IRI。 Objective:To investigate the effect and mechanisms of Phloroglucinol ( PG) on renal ische-mia and reperfusion injury (IRI).Methods:Forty-eight male Wistar rats were divided into 3 groups (16 rats per group):sham operated, saline-treated I/R (I/R), and PG-treated I/R (PG).I/R model:Af-ter removing the right kidney , renal I/R injury was induced by clamping the left renal artery for 45 min followed by reperfusion.The rats were administered with PG (30 mg/kg, intraperitoneally) or saline 15 min before renal ischemia .The blood and kidneys were harvested 6 and 24 h after reperfusion .Renal function and histologic changes of serum creatinine (SCr) and blood urea nitrogen(BUN)were assessed. Malondialdehyde (MDA),catalase (CAT),superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were measured.Nuclear factor-kapa B ( NF-κB) and caspase-3 in the kidneys were also measured.Results:SCr and BUN were (103.9 ±10.4) μmol/L and (15.2 ±1.0) mmol/L in I/R group, and (81.8 ±13.4) μmol/L and (11.5 ±1.2) mmol/L in PG group 6 h after reperfusion .SCr and BUN were (154.9 ±12.1) μmol/L and (28.1 ±1.4) mmol/L in I/R group, and (103.8 ±5.9)μmol/L和(16.0 ±1.0) mmol/L in PG group 24 h after reperfusion.PG treatment significantly attenua-ted renal dysfunction and histologic damage caused by I /R injury(P<0.05).The I/R-induced elevation in kidney MDA level decreased , where as reduced kidney SOD ,CAT and GSH-Px were increased .What is more , the apoptotic tubular cells , the levels of active caspase-3 ,and active nuclear factor kappa B dra-matically decreased after PG treatment .Conclusion:PG protects murine kidney I/R injury by suppres-sing oxidative stress , inflammation , and cell apoptosis .
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/72679
Appears in Collections:北京大学第三临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.北京大学第三医院泌尿外科,北京,100191
2.海军总医院特需医疗部,北京,100048

Recommended Citation:
李刚,张洪宪,王云鹏,等. 间苯三酚对大鼠肾缺血再灌注损伤的保护作用[J]. 北京大学学报(医学版),2015(5):743-748.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[李刚]'s Articles
[张洪宪]'s Articles
[王云鹏]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[李刚]‘s Articles
[张洪宪]‘s Articles
[王云鹏]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace