北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 内分泌科  > 期刊论文
学科主题: 内分泌
题名:
白细胞介素18对白细胞介素1的胰岛损伤效应无调节作用
其他题名: Interleukin-18 dose not modulate interleukin-1 toxic effects o n rat islet function
作者: 洪天配; 张炳勇
关键词: 白细胞介素18 ; 白细胞介素12 ; 白细胞介素1 ; Interleukin-18 Interleukin-12 Interleukin-1
刊名: 中国糖尿病杂志
发表日期: 2001
DOI: 10.3321/j.issn:1006-6187.2001.02.001
卷: 9, 期:2, 页:67-71
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的观察白细胞介素18(IL-18)对胰岛功能的效应、IL-18对IL-1β的胰岛损伤效应的影响。方法新生Wistar大鼠离体胰岛与细胞因子孵育后,观测胰岛素释放和亚硝酸盐的变化,并用逆转录-聚合酶链反应(RT-PCR)观察IL-18受体信号链(IL-18Rβ)mRNA的表达水平。结果 (1)0.625~10nmol/L基因重组小鼠(rm)IL-18孵育胰岛24h后,对累积的和葡萄糖刺激的胰岛素释放以及亚硝酸盐生成均无显著效应;(2)15pg/ml基因重组人(rh)IL-1β明显促进亚硝酸盐生成和抑制胰岛素释放,而0.625~10nmol/L rm IL-18则不影响IL-1β的上述效应;(3)rm IL-12预培养24h不能促使胰岛对10nmol/L rm或基因重组大鼠(rr)IL-18的反应性出现,也未使IL-18呈现加强IL-1β的上述效应;(4)离体胰岛即使与IL-12孵育48 h后,仍不见IL-18Rβ mRNA的表达。结论与IL-1β不同,IL-1 8在胰岛β细胞损伤中不发挥直接作用。 Objective To investigate if there is any effect of interleukin-18 (IL-18) or if IL-18 modulates IL-1β effects on islet function.Methods Insulin release and nitrite production from isolated islets of newborn Wistar rats were measured after incubation with or without cytokines.Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNA expression of the IL-18 receptor signaling chain (IL-18Rβ).Results (1) There were no significant effects of 0.625~10nmol/L of recombinant murine (rm) IL-18 alone on accumulated or glucose-challenged insulin release and nitrite production after 24h.(2) 15pg/ml of recombinant human (rh) IL-1β significantly increased nitrite production and inhibited insulin release.However,0.625~10nmol/L of rm IL-18 failed to modulate the above effects caused by IL-1β.(3) 24h rm IL-12 preincubation failed to sensitize islets to the effects of 10nmol/L of rm or recombinant rat (rr) IL-18 alone or to prime islets to IL-1β actions on insulin release and nitrite production.(4) IL-18Rβ mRNA was not expressed in isolated islets even after exposure to IL-12 for 48h.Conclusion Unlike IL-1β,IL-18 dose not play a direct role in the destruction of islet β-cell function.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/72826
Appears in Collections:北京大学第三临床医学院_内分泌科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 北京大学第三医院内分泌科,

Recommended Citation:
洪天配,张炳勇. 白细胞介素18对白细胞介素1的胰岛损伤效应无调节作用[J]. 中国糖尿病杂志,2001,9(2):67-71.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[洪天配]'s Articles
[张炳勇]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[洪天配]‘s Articles
[张炳勇]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace