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当归补血汤对骨髓移植小鼠免疫功能重建的影响
其他题名Effect of Danggui Buxue Tang on immunological reconstitution of mice following bone marrow transplantation
袁国红; 崔龙; 郝洁; 高翔; 谢蜀生
关键词当归补血汤 骨髓移植 免疫重建 Danggui Buxue Tang Bone marrow transplantation Immunological reconstitution
刊名中华微生物学和免疫学杂志
2008
DOI10.3321/j.issn:0254-5101.2008.06.009
28期:6页:510-514
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的 研究当归补血汤(DBT)对骨髓移植小鼠免疫功能重建作用的影响.方法 BALB/c小鼠一次性接受全身137Csγ线致死量照射8.5Gy,照射后0-4h内经尾静脉输注同基因骨髓细胞107/只,同时给于不同剂量DBT,每日灌胃1次,连续15d.于骨髓移植后30、60d,检测以下指标:外周血红细胞(RBC)、白细胞(WBC)计数,骨髓有核细胞计数,胸腺、脾细胞总数和相应淋巴细胞亚类,以及反映免疫细胞功能的迟发型超敏反应(DTH)、空斑形成细胞数(PFC)等.结果 经一定剂量DBT治疗的骨髓移植小鼠,其外周血中RBC、WBC计数,骨髓有核细胞计数,脾细胞和胸腺细胞总数,胸腺内各类细胞[双阴性细胞(DN)、双阳性细胞(DP)、单阳性细胞(SP)]的百分比值,与单纯骨髓移植小鼠比较差异有统计学意义(P<0.05),并且淋巴细胞的功能也得到进一步增强.到移植后60d,其各项指标进一步恢复.结论 当归补血汤可以促进骨髓移植小鼠免疫功能的恢复. Objective To study the effect of Danggui Buxue Tang (DBT) on immunological reconstitution of bone marrow transplantation (BMT) in mice. Methods BALB/c mice were irradiated by 137Cs γ once 8.5 Gy, then the mice were engrafted with bone marrow cells (107 cells/mouse) within 4 hours lethal irradiation. And the mice were fed by DBT every day for 15 days. Flow cytometry technique combined with immunological methods were performed to evaluate immunological reconstitution of BMT mice in 30 and 60 days pest-transplantation. Peripheral blood RBC and WBC were counted, and nucleated cells were assayed in recipient bone marrow. Lymphocyte numbers in thymus and periphery were counted and subpopulations of the two origins were observed respectively. Lymphocyte proliferation induced by Con A and LPS, plaque-forming cell (PFC), delayed type hypersensitivity (DTH) were also examined in 30,60 days in post-transplantation respectively. Results Compared with BMT mice, BMT mice treated with a certain dose of DBT could increase the number of peripheral blood RBC and WBC in the recipients, and also could increase that of nucleated cells significantly. BMT mice treated with DBT could increase lymphocyte numbers in thymus and periphery, and improve thymocyte subpopulations, resulting in enhancement in immune function. Conclusion DBT can enhance the immunological reconstitution of BMT mice.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/73280
专题北京大学第三临床医学院_普通外科
作者单位1.北京大学第三医院普通外科,100083
2.北京大学医学部免疫学系,100083
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袁国红,崔龙,郝洁,等. 当归补血汤对骨髓移植小鼠免疫功能重建的影响[J]. 中华微生物学和免疫学杂志,2008,28(6):510-514.
APA 袁国红,崔龙,郝洁,高翔,&谢蜀生.(2008).当归补血汤对骨髓移植小鼠免疫功能重建的影响.中华微生物学和免疫学杂志,28(6),510-514.
MLA 袁国红,et al."当归补血汤对骨髓移植小鼠免疫功能重建的影响".中华微生物学和免疫学杂志 28.6(2008):510-514.
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