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学科主题: 外科学
题名:
核因子-κB在白细胞介导大鼠移植胰腺缺血再灌注损伤中的作用
其他题名: An essential role for NF-kappa B in neutrophil-dependent ischemia/reperfusion injury following pancreaticoduodenal transplantation in rats
作者: 原春辉; 修典荣; 李丽君; 宁力; 袁国红; 张同琳
关键词: 胰腺 ; 移植 ; 再灌注损伤 ; NF-κB ; Pancreas ; Transplantation ; Reperfusion injury ; NF-κB
刊名: 中华实验外科杂志
发表日期: 2009
DOI: 10.3760/cma.j.issn.1001-9030.2009.07.021
卷: 26, 期:7, 页:873-875
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨核因子(NF)-κB在大鼠移植胰腺再灌注损伤中的作用及其可能机制.方法 应用同系大鼠异位全胰十二指肠移植模型,大鼠移植术前和术后5 min静脉注射NF-κB抑制剂脯氨酸二硫代氨基甲酸酯(ProDTC)(15 mg/kg),移植术后24 h经腹主动脉取血测定大鼠血清肿瘤坏死因子(TNF)-α、巨噬细胞炎性蛋白(MIP)-2浓度酶联免疫吸附试验(ELISA)、血糖、淀粉酶、脂肪酶水平.测定胰腺组织NF-κB p65蛋白含量(Western blot)、TNF-α mRNA、MIP-2 mRNA、细胞间黏附分子(ICAM)-1 mRNA表达逆转录-聚合酶链反应(RT-PCR)、髓过氧化物酶(MPO)活性.并进行组织学观察.结果 移植后24 h ProDTC组和对照组血清中TNF-α、MIP-2水平、胰腺组织中NF-κB p65蛋白含量、TNF-α mRNA、MIP-2 mRNA、ICAM-1 mRNA表达、MPO活性均升高,而ProDTC组水平明显低于对照组(P<0.05).移植后24 h血糖、脂肪酶水平在PmDTC组[(9.1±2.8)mmoL/L,(192±26)U/L]明显低于对照组[(13.0±3.1)mmol/L,(297±31)U/L,P<0.05].ProDTC组胰小叶间质水肿和胰小叶内中性粒细胞浸润较轻.结论 移植胰腺缺血再灌注后,NF-κB活化上调了TNF-α、MIP-2、ICAM-1表达从而增加中性粒细胞浸润,外源性应用NF-κB抑制剂ProDTC可以减轻移植胰腺缺血再灌注损伤. Objective To investigate the effects of nuclear factor-κB (NF-κB) on reperfusion injury following pancreaticoduodenal transplantation in rots. Methods The (homologous) male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was used. The proline dithiocarbamates (PmDTC) -treated rats received the intravenous injection of ProDTC ( 15 mg/kg) 5 min before and after operation. The content of NF-κBp65 in pancreas nuclear extracts (Westem blot), the expression of TNF-a, MIP-2 and ICAM-1 transcripts (RT-PCR), the concentrations of serum TNF-α and MIP-2 (ELISA), blood sugar (BG) and serum concentrations of amylase and lipase,and pancreas myeloperoxidase (MPO) were analyzed. Results The content of NF-κBp65, the transcription of TNF-α, MIP-2 and ICAM-1, and the release of serum TNF-α and MIP-2 were significantly increased after transplantation both in the untreated and ProDTC-treated rats,while the levels in the ProDTC-treated group were obviously higher than in the untreated group. Administration of ProDTC resulted in a statistically significant decrease in blood sugar [ (9.1±2.8) mmol/L] and serum concentration of lipase [ (192±26) U/L] against the levels in the untreated rats [(13.0±3.1) mmol/L,(297±31) U/L,P<0.05]. Conclusion NF-κB inhibitor ProDTC can decrease the amount of NF-κB, and inhibit the elevation of TNF-α, MIP-2 and ICAM-1 and early neutrophil accumulation in the pancreas. NF-κB inhibitor ProDTC has protective effects on the pan-ereatic ischemia/reperfusion injury after pancreaticoduodenal transplantation.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/73839
Appears in Collections:北京大学第三临床医学院_普通外科_期刊论文

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作者单位: 1.北京大学第三医院普通外科,100083
2.北京协和医院

Recommended Citation:
原春辉,修典荣,李丽君,等. 核因子-κB在白细胞介导大鼠移植胰腺缺血再灌注损伤中的作用[J]. 中华实验外科杂志,2009,26(7):873-875.
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