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学科主题: 临床医学
题名:
成人骨髓间充质干细胞体外向视网膜细胞的诱导分化
其他题名: Human bone marrow-derived mesenchymal stem cells differentiate into retinal cells in vitro
作者: 俞海燕; 吴文涛; 王薇; 张纯
关键词: 骨髓 ; 间充质干细胞 ; 视网膜 ; 体外诱导分化 ; 免疫荧光染色 ; Rhodopsin ; bone marrow ; mesenchymal stem cells ; retina ; cellular differentiation in vitro ; immunofluores-ence staining ; Rhodopsin
刊名: 中国药理学通报
发表日期: 2014
DOI: 10.3969/j.issn.1001-1978.2014.06.011
期: 6, 页:787-790,791
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的:研究取材于成人骨髓的间充质干细胞在一定诱导条件下向视网膜神经细胞的分化。方法成人骨髓经密度梯度离心得到的细胞,根据其高黏附特性体外培养获得间充质干细胞。利用流式细胞仪分析其细胞表型,在体外诱导使其向视网膜神经细胞分化并用免疫荧光法进行鉴定。结果从骨髓中分离培养的细胞具有成纤维细胞样形态,贴壁生长,表型相对均一,表面标志为CD90、CD44、CD147阳性;而CD34、CD38、CD45、CD14、HLA-DR阴性。体外诱导后可以得到表达nestin(神经干细胞标志物)、GFAP(神经胶质细胞标志物)和Rhodopsin(视网膜光感受器细胞标志物)阳性的细胞。结论从人骨髓中分离培养得到的间充质干细胞具有向视网膜神经细胞分化的潜能。 Aim To study the differentiation of human bone marrow-derived mesenchymal stem cells ( HM-SCs) into retinal cells in vitro. Methods HMSCs were isolated from human bone marrow after Ficoll den-sity gradient centrifugation. The adherent cells after at least 5 passages were used for study. Immunopheno-type of the cells was analysed by flow cytometer, and cellular differentiation was identified by immunofluores-cence labeling technique. Results The target cells derived from human bone marrow adhered to the plate with fibroblastic-like morphology, whose surface mark-ers were similar to mesenchymal stem cells. Major cells
were positive for CD90 , CD44 , CD147 , while they were all negative for CD34, CD45, HLA-DR. In the differentiation study, HMSCs cultured in induced me-dium can differentiate into nestin ( neural stem cell ) -positive cell, GFAP ( glial fibrillary acidic protein ) -positive glial cells and retina-specific neurons express-ing Rhodopsin with CD90 ( mesenchymal stem cells )-negative. Conclusion HMSCs have the ability to dif-ferentiate into retinal neural cells in vitro.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/74079
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.北京大学第三医院 临床干细胞研究中心,北京,100191
2.北京大学第三医院 眼科中心,北京,100191

Recommended Citation:
俞海燕,吴文涛,王薇,等. 成人骨髓间充质干细胞体外向视网膜细胞的诱导分化[J]. 中国药理学通报,2014(6):787-790,791.
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