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学科主题: 内分泌
题名:
Ghrelin抑制胰岛β细胞胰岛素释放的机制探讨
其他题名: The inhibitory effect of ghrelin on insulin release of mouse islet β-cell line and its mechanism
作者: 李琳; 吴永华; 洪天配; 邓正照
关键词: Ghrelin ; 胰岛β细胞 ; 胰岛素 ; Ghrelin ; Islet β-cells ; Insulin
刊名: 中国糖尿病杂志
发表日期: 2006
DOI: 10.3321/j.issn:1006-6187.2006.06.021
卷: 14, 期:6, 页:452-454
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨ghrelin对小鼠胰腺β细胞株NIT-1细胞胰岛素释放的影响及其作用机制. 方法 NIT-1细胞与不同浓度ghrelin和高浓度葡萄糖孵育后,放免法测定上清液胰岛素含量,RT-PCR法检测葡萄糖转运子2(GluT2)、胰十二指肠同源盒-1(PDX-1)、含两个跨膜区的内向整流钾通道(Kir6.2)及磺酰脲受体1(SUR-1) 等基因的表达.NIT-1细胞与不同浓度ghrelin孵育不同时间后,MTT法检测细胞增殖情况. 结果 (1)10-9mol/L至10-7mol/L ghrelin呈剂量依赖性抑制NIT-1细胞高浓度葡萄糖刺激的胰岛素释放;(2)10-7mol/L ghrelin显著降低Kir6.2 mRNA的表达,但对GluT2、PDX-1及SUR-1 mRNA的表达无明显的效应;(3)Ghrelin对NIT-1细胞的增殖无显著影响. 结论 Ghrelin可抑制胰岛β细胞高浓度葡萄糖刺激的胰岛素释放,该效应可能是由于下调ATP敏感性钾通道组成成分Kir6.2基因的表达所致. Objective To explore the effect of ghrelin on insulin release of mouse pancreatic islet β-cell line(NIT-1 cells) and its probable mechanism.Methods NIT-1 cells were incubated in high-glucose DMEM with ghrelin.Then,the media was sampled for the assay of insulin by RIA.The(mRNA) expressions of glucose transporter 2(GluT2),pancreatic-duodenal homeobox-1(PDX-1),inwardly rectifying potassium channel with two transmembrane regions(Kir6.2) and sulphonylurea receptor 1(SUR-1) in the cells were detected by using RT-PCR.The cell proliferation was determined by MTT assay.Results(1) 10-9mol/L to 10-7mol/L of ghrelin inhibited dose-dependently the high-glucose challenged insulin release of the NIT-1 cells.(2) The mRNA expression of Kir6.2,but not GluT2,PDX-1and SUR-1,was down-regulated by 10-7mol/L of ghrelin.(3) Ghrelin had no effect on proliferation of the cells.Conclusions Ghrelin inhibits high-glucose induced insulin secretion of the islet β-cells.This effect may be secondary to the down-regulation for the expression of Kir6.2,(a component) of ATP-sensitive potassium channel.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/74602
Appears in Collections:北京大学第三临床医学院_内分泌科_期刊论文

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作者单位: 100083,北京大学第三医院内分泌科,北京大学糖尿病中心

Recommended Citation:
李琳,吴永华,洪天配,等. Ghrelin抑制胰岛β细胞胰岛素释放的机制探讨[J]. 中国糖尿病杂志,2006,14(6):452-454.
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