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学科主题: 内分泌
题名:
干扰素γ促进胰岛β细胞中白细胞介素18和白细胞介素1β转换酶的表达
其他题名: Interferon-γ up-regulates interleukin-18 and caspase-1 expression in islet β-cells
作者: 洪天配; Thomas, MANDRUP-POULSEN
关键词: 干扰素γ ; 白细胞介素18 ; 白细胞介素1β转换酶 ; Interleukin 1β converting enzyme
刊名: 中国糖尿病杂志
发表日期: 2002
DOI: 10.3321/j.issn:1006-6187.2002.04.004
卷: 10, 期:4, 页:205-208
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的探讨白细胞介素18(IL-18)和IL-1β转换酶(caspase-1)在胰岛β细胞的表达状况及其调控机制. 方法大鼠胰岛素瘤(RIN)细胞、FACS纯化的大鼠胰岛β细胞、大鼠离体胰岛和干扰素调节因子1(IRF-1)基因敲除小鼠的离体胰岛与细胞因子孵育后,用逆转录-聚合酶链反应(RT-PCR)方法检测IL-18和caspase-1 mRNA的表达,用Western blot方法检测IL-18蛋白的表达. 结果 (1)干扰素γ(IFN-γ)可增强IL-18 mRNA在RIN细胞、纯化大鼠β细胞和大鼠离体胰岛中的表达,该效应在IRF-1基因敲除小鼠胰岛中未见明显变化;(2)IFN-γ显著增强caspase-1 mRNA在RIN细胞和大鼠离体胰岛中的表达,该效应在IRF-1基因敲除小鼠胰岛中完全消失;(3)在RIN细胞的培养上清液和细胞裂解物中未能检测到IL-18蛋白. 结论 IFN-γ上调IL-18和caspase-1在胰岛β细胞中的表达,IL-18的表达不受转录因子IRF-1的影响,caspase-1的表达呈IRF-1依赖性. Objective To investigate whether interleukin 18 (IL 18) and IL 1β converting enzyme (ICE, caspase 1) is expressed in islet β cells and the mechanism of their expression control. Methods Rat insulinoma (RIN) cells, FACS purified rat β cells, isolated rat islets, and islets isolated from interferon regulatory factor 1 (IRF 1) gene knockout mice were incubated with or without cytokines. Reverse transcription polymerase chain reaction (RT PCR) was used to quantitate IL 18 and caspase 1 mRNA expression. IL 18 protein expression was detected by using Western blot. Results (1)Interferon γ (IFN γ) increased IL 18 mRNA expression in RIN cells, purified rat β cells, and intact rat islets, and the effect was unchanged in IRF 1 knockout mouse islets. (2) IFN γ highly up regulated caspase 1 mRNA expression in RIN cells and intact rat islets, but the expression was abolished in IRF 1 knockout mouse islets. (3) IL 18 protein was undetectable in lysates and supernatants of RIN cells. Conclusion IFN γ up regulates IL 18 and caspase 1 expression in islet β cells. IL 18 expression is not dependent on a transcription factor IRF 1, while caspase 1 expression is in an IRF 1 dependent way.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/74604
Appears in Collections:北京大学第三临床医学院_内分泌科_期刊论文

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作者单位: 1.100083,北京大学第三医院内分泌科
2.丹麦Steno糖尿病中心

Recommended Citation:
洪天配,Thomas, MANDRUP-POULSEN. 干扰素γ促进胰岛β细胞中白细胞介素18和白细胞介素1β转换酶的表达[J]. 中国糖尿病杂志,2002,10(4):205-208.
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