北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 内分泌科  > 期刊论文
学科主题: 内分泌
题名:
依法克生对胰岛β细胞表达Ras同源物的调节作用
其他题名: Effect of efaroxan on the expression of a Ras homologue in islet β cells
作者: 高洪伟; NG, Morgan
关键词: 依法克生 ; KU14R ; Rhes ; β细胞 ; β cell
刊名: 中国糖尿病杂志
发表日期: 2002
DOI: 10.3321/j.issn:1006-6187.2002.03.008
卷: 10, 期:3, 页:155-158
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的观察Ras同源物(Rhes)在胰岛β细胞中的表达以及依法克生(efaroxan)对Rhes表达的影响. 方法用逆转录-聚合酶链反应(RT-PCR)观察Rhes在克隆大鼠胰岛素分泌细胞(RINm5F和BRIN BD11细胞)以及离体大鼠完整胰岛中的表达. BRIN BD11细胞经依法克生24 h预处理后,观察依法克生和(或)依法克生的结构类似物(KU14R)对胰岛素释放的影响. 结果 (1) RINm5F、BRIN BD11细胞和离体完整大鼠胰岛均表达Rhes.(2) 依法克生对Rhes的表达有降调节作用.KU14R对Rhes的表达无影响,但能完全阻断依法克生对Rhes表达的降调节作用.(3) 依法克生可诱导BRIN BD11细胞对其刺激胰岛分泌作用产生脱敏效应.(4)KU14R可刺激BRIB BD11细胞分泌胰岛素. 结论胰岛β细胞可表达Rhes.Rhes是受咪唑啉类调控的分子,可能参与调节依法克生诱导的β细胞胰岛素释放. Objective To investigate whether a Ras homologue, Rhes, is expressed and the effect of efaroxan on Rhes expression in islet β cells. Methods Reverse transcription and polymerese chain reaction(RT PCR)was employed to detect Rhes transcript in clonal rat insulin releasing cells (RINm5F and BRIN BD11 cells) and intact isolated rat islets. Insulin release was measured after exposure to efaroxan and/or KU14R for 2hr under the condition that BRIN BD11 cells were precultured with or without efaroxna for 24 hr. Results (1) Rhes is expressed in RINm5F cells and BRIN BD11 cells and isolated rat islets. (2) Rhes expression was down regulated by efaroxan. KU14R, a structurally related molecule to efaroxan, had no effect on Rhes expression, however, it completely blocked the down regulation of Rhes expression induced by efaroxan. (3) Efaroxan induced a state of functional desensitization in clonal BRIN BD11 cells after 24 h exposure. (4)Unlike being in isolated human and rat islets, KU14R itself stimulated insulin secretion in clonal BRIN BD11 cells. Conclusion Rhes is expressed in islet β cells. Rhes represents an imidazoline regulated molecule that could be involved in mediating the insulin secretory response to efaroxan.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/74605
Appears in Collections:北京大学第三临床医学院_内分泌科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.100083,北京大学第三医院内分泌科
2.英国Keele大学生命科学院

Recommended Citation:
高洪伟,NG, Morgan. 依法克生对胰岛β细胞表达Ras同源物的调节作用[J]. 中国糖尿病杂志,2002,10(3):155-158.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[高洪伟]'s Articles
[NG, Morgan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[高洪伟]‘s Articles
[NG, Morgan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace